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231225s2018 xx |||||o 00| ||eng c |
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|a 10.1002/adma.201801632
|2 doi
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|a pubmed24n0952.xml
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|a (DE-627)NLM285824775
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|a (NLM)29938845
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|a DE-627
|b ger
|c DE-627
|e rakwb
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|a eng
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| 100 |
1 |
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|a Atwater, Jordyn
|e verfasserin
|4 aut
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| 245 |
1 |
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|a Combinatorial Synthesis of Macromolecular Arrays by Microchannel Cantilever Spotting (µCS)
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|c 2018
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
|b c
|2 rdamedia
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| 338 |
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|a ƒa Online-Ressource
|b cr
|2 rdacarrier
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| 500 |
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|a Date Completed 25.09.2018
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|a Date Revised 30.09.2020
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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|a © 2018 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
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|a Surface-bound microarrays of multiple oligo- and macromolecules (e.g., peptides, DNA) offer versatile options in biomedical applications like drug screening, DNA analysis, or medical diagnostics. Combinatorial syntheses of these molecules in situ can save significant resources in regard to processing time and material use. Furthermore, high feature densities are needed to enable high-throughput and low sample volumes as generally regarded in combinatorial chemistry. Here, a scanning-probe-lithography-based approach for the combinatorial in situ synthesis of macromolecules is presented in microarray format. Feature sizes below 40 µm allow for the creation of high-density arrays with feature densities of 62 500 features per cm2 . To demonstrate feasibility of this approach for biomedical applications, a multiplexed array of functional protein tags (HA- and FLAG-tag) is synthesized, and selective binding of respective epitope recognizing antibodies is shown. This approach uses only small amounts of base chemicals for synthesis and can be further parallelized, therefore, opening up a route to flexible, highly dense, and cost-effective microarrays
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|a Journal Article
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|a combinatorial chemistry
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|a high-throughput screening
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|a microarrays
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|a peptides
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|a scanning probe lithography
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|a Antibodies
|2 NLM
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| 650 |
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|a Epitopes
|2 NLM
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| 650 |
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|a Hemagglutinins, Viral
|2 NLM
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| 650 |
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|a Peptides
|2 NLM
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| 650 |
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|a Polymers
|2 NLM
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| 700 |
1 |
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|a Mattes, Daniela S
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Streit, Bettina
|e verfasserin
|4 aut
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| 700 |
1 |
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|a von Bojničić-Kninski, Clemens
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Loeffler, Felix F
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Breitling, Frank
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Fuchs, Harald
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Hirtz, Michael
|e verfasserin
|4 aut
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| 773 |
0 |
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|i Enthalten in
|t Advanced materials (Deerfield Beach, Fla.)
|d 1998
|g 30(2018), 31 vom: 30. Aug., Seite e1801632
|w (DE-627)NLM098206397
|x 1521-4095
|7 nnns
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| 773 |
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|g volume:30
|g year:2018
|g number:31
|g day:30
|g month:08
|g pages:e1801632
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| 856 |
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|u http://dx.doi.org/10.1002/adma.201801632
|3 Volltext
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|d 30
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|e 31
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|c 08
|h e1801632
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