High V-PPase activity is beneficial under high salt loads, but detrimental without salinity

High V-PPase activity is beneficial under high salt loads, but detrimental without salinity

© 2018 The Authors. New Phytologist © 2018 New Phytologist Trust.

Bibliographische Detailangaben
Veröffentlicht in:The New phytologist. - 1979. - 219(2018), 4 vom: 09. Sept., Seite 1421-1432
1. Verfasser: Graus, Dorothea (VerfasserIn)
Weitere Verfasser: Konrad, Kai R, Bemm, Felix, Patir Nebioglu, Meliha Görkem, Lorey, Christian, Duscha, Kerstin, Güthoff, Tilman, Herrmann, Johannes, Ferjani, Ali, Cuin, Tracey Ann, Roelfsema, M Rob G, Schumacher, Karin, Neuhaus, H Ekkehard, Marten, Irene, Hedrich, Rainer
Format: Online-Aufsatz
Sprache:English
Veröffentlicht: 2018
Zugriff auf das übergeordnete Werk:The New phytologist
Schlagworte:Journal Article Research Support, Non-U.S. Gov't cell death plasma membrane voltage proton pump currents salt vacuolar pH vacuolar proton-ATPase (V-ATPase) vacuolar proton-pyrophosphatase (V-PPase) Diphosphates mehr... Isoenzymes Proton Pumps Protons Sodium Chloride 451W47IQ8X Vacuolar Proton-Translocating ATPases EC 3.6.1.- Inorganic Pyrophosphatase EC 3.6.1.1
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520 |a The membrane-bound proton-pumping pyrophosphatase (V-PPase), together with the V-type H+ -ATPase, generates the proton motive force that drives vacuolar membrane solute transport. Transgenic plants constitutively overexpressing V-PPases were shown to have improved salinity tolerance, but the relative impact of increasing PPi hydrolysis and proton-pumping functions has yet to be dissected. For a better understanding of the molecular processes underlying V-PPase-dependent salt tolerance, we transiently overexpressed the pyrophosphate-driven proton pump (NbVHP) in Nicotiana benthamiana leaves and studied its functional properties in relation to salt treatment by primarily using patch-clamp, impalement electrodes and pH imaging. NbVHP overexpression led to higher vacuolar proton currents and vacuolar acidification. After 3 d in salt-untreated conditions, V-PPase-overexpressing leaves showed a drop in photosynthetic capacity, plasma membrane depolarization and eventual leaf necrosis. Salt, however, rescued NbVHP-hyperactive cells from cell death. Furthermore, a salt-induced rise in V-PPase but not of V-ATPase pump currents was detected in nontransformed plants. The results indicate that under normal growth conditions, plants need to regulate the V-PPase pump activity to avoid hyperactivity and its negative feedback on cell viability. Nonetheless, V-PPase proton pump function becomes increasingly important under salt stress for generating the pH gradient necessary for vacuolar proton-coupled Na+ sequestration 
650 4 |a Journal Article 
650 4 |a Research Support, Non-U.S. Gov't 
650 4 |a cell death 
650 4 |a plasma membrane voltage 
650 4 |a proton pump currents 
650 4 |a salt 
650 4 |a vacuolar pH 
650 4 |a vacuolar proton-ATPase (V-ATPase) 
650 4 |a vacuolar proton-pyrophosphatase (V-PPase) 
650 7 |a Diphosphates  |2 NLM 
650 7 |a Isoenzymes  |2 NLM 
650 7 |a Proton Pumps  |2 NLM 
650 7 |a Protons  |2 NLM 
650 7 |a Sodium Chloride  |2 NLM 
650 7 |a 451W47IQ8X  |2 NLM 
650 7 |a Vacuolar Proton-Translocating ATPases  |2 NLM 
650 7 |a EC 3.6.1.-  |2 NLM 
650 7 |a Inorganic Pyrophosphatase  |2 NLM 
650 7 |a EC 3.6.1.1  |2 NLM 
700 1 |a Konrad, Kai R  |e verfasserin  |4 aut 
700 1 |a Bemm, Felix  |e verfasserin  |4 aut 
700 1 |a Patir Nebioglu, Meliha Görkem  |e verfasserin  |4 aut 
700 1 |a Lorey, Christian  |e verfasserin  |4 aut 
700 1 |a Duscha, Kerstin  |e verfasserin  |4 aut 
700 1 |a Güthoff, Tilman  |e verfasserin  |4 aut 
700 1 |a Herrmann, Johannes  |e verfasserin  |4 aut 
700 1 |a Ferjani, Ali  |e verfasserin  |4 aut 
700 1 |a Cuin, Tracey Ann  |e verfasserin  |4 aut 
700 1 |a Roelfsema, M Rob G  |e verfasserin  |4 aut 
700 1 |a Schumacher, Karin  |e verfasserin  |4 aut 
700 1 |a Neuhaus, H Ekkehard  |e verfasserin  |4 aut 
700 1 |a Marten, Irene  |e verfasserin  |4 aut 
700 1 |a Hedrich, Rainer  |e verfasserin  |4 aut 
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773 1 8 |g volume:219  |g year:2018  |g number:4  |g day:09  |g month:09  |g pages:1421-1432 
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