EMR, a cytosolic-abundant ring finger E3 ligase, mediates ER-associated protein degradation in Arabidopsis

EMR, a cytosolic-abundant ring finger E3 ligase, mediates ER-associated protein degradation in Arabidopsis

© 2018 The Authors. New Phytologist © 2018 New Phytologist Trust.

Bibliographische Detailangaben
Veröffentlicht in:The New phytologist. - 1979. - 220(2018), 1 vom: 22. Okt., Seite 163-177
1. Verfasser: Park, Joung Hun (VerfasserIn)
Weitere Verfasser: Kang, Chang Ho, Nawkar, Ganesh M, Lee, Eun Seon, Paeng, Seol Ki, Chae, Ho Byoung, Chi, Yong Hun, Kim, Woe Yeon, Yun, Dae-Jin, Lee, Sang Yeol
Format: Online-Aufsatz
Sprache:English
Veröffentlicht: 2018
Zugriff auf das übergeordnete Werk:The New phytologist
Schlagworte:Journal Article Research Support, Non-U.S. Gov't Arabidopsis thaliana E3 ligase brassinosteroid (BR) signaling endoplasmic reticulum (ER) stress endoplasmic reticulum-associated degradation (ERAD) misfolded proteins Arabidopsis Proteins Brassinosteroids mehr... RNA, Messenger AT4G26400 protein, Arabidopsis EC 2.3.- Acyltransferases UBC32 protein, Arabidopsis EC 2.3.2.23 Ubiquitin-Conjugating Enzymes Ubiquitin-Protein Ligases EC 2.3.2.27
Beschreibung
Zusammenfassung:© 2018 The Authors. New Phytologist © 2018 New Phytologist Trust.
Investigation of the endoplasmic reticulum-associated degradation (ERAD) system in plants led to the identification of ERAD-mediating RING finger protein (EMR) as a plant-specific ERAD E3 ligase from Arabidopsis. EMR was significantly up-regulated under endoplasmic reticulum (ER) stress conditions. The EMR protein purified from bacteria displayed high E3 ligase activity, and tobacco leaf-produced EMR mediated mildew resistance locus O-12 (MLO12) degradation in a proteasome-dependent manner. Subcellular localization and coimmunoprecipitation analyses showed that EMR forms a complex with ubiquitin-conjugating enzyme 32 (UBC32) as a cytosolic interaction partner. Mutation of EMR and RNA interference (RNAi) increased the tolerance of plants to ER stress. EMR RNAi in the bri1-5 background led to partial recovery of the brassinosteroid (BR)-insensitive phenotypes as compared with the original mutant plants and increased ER stress tolerance. The presented results suggest that EMR is involved in the plant ERAD system that affects BR signaling under ER stress conditions as a novel Arabidopsis ring finger E3 ligase mainly present in cytosol while the previously identified ERAD E3 components are typically membrane-bound proteins
Beschreibung:Date Completed 01.10.2019
Date Revised 30.09.2020
published: Print-Electronic
Citation Status MEDLINE
ISSN:1469-8137
DOI:10.1111/nph.15279