DNA assembly standards : Setting the low-level programming code for plant biotechnology

Bibliographische Detailangaben
Veröffentlicht in:	Plant science : an international journal of experimental plant biology. - 1985. - 273(2018) vom: 31. Aug., Seite 33-41
1. Verfasser:	Vazquez-Vilar, Marta (VerfasserIn)
Weitere Verfasser:	Orzaez, Diego, Patron, Nicola
Format:	Online-Aufsatz
Sprache:	English
Veröffentlicht:	2018
Zugriff auf das übergeordnete Werk:	Plant science : an international journal of experimental plant biology
Schlagworte:	Journal Article Review Biotechnology CRISPR DNA assembly Genome engineering Laboratory automation Molecular cloning Synthetic biology DNA, Plant

Beschreibung
Zusammenfassung:	Copyright © 2018 Elsevier B.V. All rights reserved. Synthetic Biology is defined as the application of engineering principles to biology. It aims to increase the speed, ease and predictability with which desirable changes and novel traits can be conferred to living cells. The initial steps in this process aim to simplify the encoding of new instructions in DNA by establishing low-level programming languages for biology. Together with advances in the laboratory that allow multiple DNA molecules to be efficiently assembled together into a desired order in a single step, this approach has simplified the design and assembly of multigene constructs and has even facilitated the automated construction of synthetic chromosomes. These advances and technologies are now being applied to plants, for which there are a growing number of software and wetware tools for the design, construction and delivery of DNA molecules and for the engineering of endogenous genes. Here we review the efforts of the past decade that have established synthetic biology workflows and tools for plants and discuss the constraints and bottlenecks of this emerging field
Beschreibung:	Date Completed 24.09.2018 Date Revised 09.01.2021 published: Print-Electronic Citation Status MEDLINE
ISSN:	1873-2259
DOI:	10.1016/j.plantsci.2018.02.024