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231225s2018 xx |||||o 00| ||eng c |
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|a 10.1016/j.plaphy.2018.06.004
|2 doi
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|a pubmed24n0951.xml
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|a (DE-627)NLM285392832
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|a (NLM)29894859
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|a (PII)S0981-9428(18)30255-9
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|a DE-627
|b ger
|c DE-627
|e rakwb
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|a eng
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| 100 |
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|a Banchi, Elisa
|e verfasserin
|4 aut
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|a Relation between water status and desiccation-affected genes in the lichen photobiont Trebouxia gelatinosa
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|c 2018
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
|b c
|2 rdamedia
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|a ƒa Online-Ressource
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|2 rdacarrier
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|a Date Completed 12.02.2019
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|a Date Revised 30.09.2020
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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|a Copyright © 2018 Elsevier Masson SAS. All rights reserved.
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|a The relation between water status and expression profiles of desiccation -related genes has been studied in the desiccation tolerant (DT) aeroterrestrial green microalga Trebouxia gelatinosa, a common lichen photobiont. Algal colonies were desiccated in controlled conditions and during desiccation water content (WC) and water potential (Ψ) were measured to find the turgor loss point (Ψtlp). Quantitative real-time PCR was performed to measure the expression of ten genes related to photosynthesis, antioxidant defense, expansins, heat shock proteins (HSPs), and desiccation related proteins in algal colonies collected during desiccation when still at full turgor (WC > 6 g H2O g-1 dry weight), immediately before and after Ψtlp (-4 MPa; WC ∼ 1 g H2O g-1 dry weight) and before and after complete desiccation (WC < 0.01 g H2O g-1 dry weight), quantifying the HSP70 protein levels by immunodetection. Our analysis showed that the expression of eight out of ten genes changed immediately before and after Ψtlp. Interestingly, the expression of five out of ten genes changed also before complete desiccation, i.e. between 0.2 and 0.01 g H2O g-1 dry weight. However, the HSP70 protein levels were not affected by changes in water status. The study provides new evidences of the link between the loss of turgor and the expression of genes related to the desiccation tolerance of T. gelatinosa, suggesting the former as a signal triggering inducible mechanisms
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|a Journal Article
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|a DRPs
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|a Green microalga
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|a HSP70
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|a Turgor loss
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|a Water content
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|a Water potential
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|a HSP70 Heat-Shock Proteins
|2 NLM
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|a Water
|2 NLM
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|a 059QF0KO0R
|2 NLM
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|a Chlorophyll A
|2 NLM
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|a YF5Q9EJC8Y
|2 NLM
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| 700 |
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|a Candotto Carniel, Fabio
|e verfasserin
|4 aut
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|a Montagner, Alice
|e verfasserin
|4 aut
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|a Petruzzellis, Francesco
|e verfasserin
|4 aut
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|a Pichler, Gregor
|e verfasserin
|4 aut
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|a Giarola, Valentino
|e verfasserin
|4 aut
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|a Bartels, Dorothea
|e verfasserin
|4 aut
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|a Pallavicini, Alberto
|e verfasserin
|4 aut
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|a Tretiach, Mauro
|e verfasserin
|4 aut
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| 773 |
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|i Enthalten in
|t Plant physiology and biochemistry : PPB
|d 1991
|g 129(2018) vom: 15. Aug., Seite 189-197
|w (DE-627)NLM098178261
|x 1873-2690
|7 nnns
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| 773 |
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|g volume:129
|g year:2018
|g day:15
|g month:08
|g pages:189-197
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|u http://dx.doi.org/10.1016/j.plaphy.2018.06.004
|3 Volltext
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