Association of high 5-hydroxymethylcytosine levels with Ten Eleven Translocation 2 overexpression and inflammation in Sjögren's syndrome patients

Association of high 5-hydroxymethylcytosine levels with Ten Eleven Translocation 2 overexpression and inflammation in Sjögren's syndrome patients

Copyright © 2018 Elsevier Inc. All rights reserved.

Bibliographische Detailangaben
Veröffentlicht in:Clinical immunology (Orlando, Fla.). - 1999. - 196(2018) vom: 01. Nov., Seite 85-96
1. Verfasser: Lagos, Carolina (VerfasserIn)
Weitere Verfasser: Carvajal, Patricia, Castro, Isabel, Jara, Daniela, González, Sergio, Aguilera, Sergio, Barrera, María-José, Quest, Andrew F G, Bahamondes, Verónica, Molina, Claudio, Urzúa, Ulises, Hermoso, Marcela A, Leyton, Cecilia, González, María-Julieta
Format: Online-Aufsatz
Sprache:English
Veröffentlicht: 2018
Zugriff auf das übergeordnete Werk:Clinical immunology (Orlando, Fla.)
Schlagworte:Journal Article Research Support, Non-U.S. Gov't 5-hydroxymethylcytosine Global DNA methylation Pro-inflammatory cytokines Sjögren's syndrome TET2 Cytokines DNA-Binding Proteins DNMT3A protein, human mehr... MECP2 protein, human Methyl-CpG-Binding Protein 2 Proto-Oncogene Proteins 1123-95-1 5-Methylcytosine 6R795CQT4H Mixed Function Oxygenases EC 1.- TET1 protein, human TET3 protein, human Dioxygenases EC 1.13.11.- TET2 protein, human DNA (Cytosine-5-)-Methyltransferase 1 EC 2.1.1.37 DNA (Cytosine-5-)-Methyltransferases DNA Methyltransferase 3A DNMT1 protein, human Cytidine Deaminase EC 3.5.4.5
Beschreibung
Zusammenfassung:Copyright © 2018 Elsevier Inc. All rights reserved.
Here, we determined the 5-hydroxymethylcytosine (5hmC), 5-methylcytosine (5mC), Ten Eleven Translocation (TETs), and DNA methyltransferases (DNMTs) levels in epithelial and inflammatory cells of labial salivary glands (LSG) from Sjögren's syndrome (SS)-patients and the effect of cytokines on HSG cells. LSG from SS-patients, controls and HSG cells incubated with cytokines were analysed. Levels of 5mC, 5hmC, DNMTs, TET2 and MeCP2 were assessed by immunofluorescence. In epithelial cells from SS-patients, an increase in TET2, 5hmC and a decrease in 5mC and MeCP2 were observed, additionally, high levels of 5mC and DNMTs and low levels of 5hmC were detected in inflammatory cells. Cytokines increased TET2 and 5hmC and decreased 5mC levels. Considering that the TET2 gene.promoter contains response elements for transcription factors activated by cytokines, together to in vitro results suggest that changes in DNA hydroxymethylation, resulting from altered levels of TET2 are likely to be relevant in the Sjögren's syndrome etiopathogenesis
Beschreibung:Date Completed 14.10.2019
Date Revised 13.12.2023
published: Print-Electronic
Citation Status MEDLINE
ISSN:1521-7035
DOI:10.1016/j.clim.2018.06.002