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231225s2018 xx |||||o 00| ||eng c |
| 024 |
7 |
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|a 10.1111/jfd.12828
|2 doi
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| 028 |
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2 |
|a pubmed24n0950.xml
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|a (DE-627)NLM28527015X
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|a (NLM)29882295
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|a DE-627
|b ger
|c DE-627
|e rakwb
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| 041 |
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|a eng
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| 100 |
1 |
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|a Nguyen, Hai Trong
|e verfasserin
|4 aut
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| 245 |
1 |
0 |
|a Enhanced immune responses and effectiveness of refined outer membrane protein vaccines against Vibrio harveyi in orange-spotted grouper (Epinephelus coioides)
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| 264 |
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1 |
|c 2018
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| 336 |
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|a Text
|b txt
|2 rdacontent
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| 337 |
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|a ƒaComputermedien
|b c
|2 rdamedia
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| 338 |
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|a ƒa Online-Ressource
|b cr
|2 rdacarrier
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| 500 |
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|a Date Completed 30.01.2019
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| 500 |
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|a Date Revised 30.09.2020
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| 500 |
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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| 520 |
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|a © 2018 John Wiley & Sons Ltd.
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|a Vibriosis is a severe infection occurring in many commercially important marine fish species. In this study, vaccines containing Vibrio harveyi recombinant outer membrane protein K (rOmpK), outer membrane protein U (rOmpU) and rOmpK-OmpU fusion protein in addition to the metabolizable MontanideTM ISA 763 A VG adjuvant were developed and evaluated in the orange-spotted grouper. The results indicate that recombinant V. harveyi protein-based vaccines resulted in a remarkably higher expression of IL-1β and IL-8 at 24 hr, and greater antibody production, as early as 2 weeks postimmunization. Notably, enhanced immune responses and significant protective efficacy against V. harveyi infections were observed in the fusion protein vaccine-injected fishes with relative per cent survival value of 81.8%. Additionally, the rOmpK-OmpU antisera presented a high bactericidal effect on not only V. harveyi, but also Vibrio parahaermolyticus and Vibrio alginolyticus. Our results demonstrated that the fusion protein rOmpK-OmpU was an effective vaccine candidate that exhibited potentially great versatility for controlling vibrio infections
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| 650 |
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4 |
|a Journal Article
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| 650 |
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4 |
|a Vibrio harveyi
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| 650 |
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4 |
|a ISA 763 A VG
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| 650 |
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4 |
|a fusion protein
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| 650 |
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4 |
|a vaccine
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| 650 |
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4 |
|a vibriosis
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| 650 |
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7 |
|a Adjuvants, Immunologic
|2 NLM
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| 650 |
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7 |
|a Antibodies, Bacterial
|2 NLM
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| 650 |
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7 |
|a Bacterial Outer Membrane Proteins
|2 NLM
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| 650 |
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7 |
|a Bacterial Vaccines
|2 NLM
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| 650 |
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7 |
|a Fish Proteins
|2 NLM
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| 650 |
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7 |
|a Interleukin-1beta
|2 NLM
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| 650 |
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7 |
|a Interleukin-8
|2 NLM
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| 650 |
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7 |
|a Recombinant Proteins
|2 NLM
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| 700 |
1 |
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|a Nguyen, Thuy Thi Thu
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Chen, Yan-Chun
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Vu-Khac, Hung
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Wang, Pei-Chi
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Chen, Shih-Chu
|e verfasserin
|4 aut
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| 773 |
0 |
8 |
|i Enthalten in
|t Journal of fish diseases
|d 1998
|g 41(2018), 9 vom: 08. Sept., Seite 1349-1358
|w (DE-627)NLM098166034
|x 1365-2761
|7 nnns
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| 773 |
1 |
8 |
|g volume:41
|g year:2018
|g number:9
|g day:08
|g month:09
|g pages:1349-1358
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| 856 |
4 |
0 |
|u http://dx.doi.org/10.1111/jfd.12828
|3 Volltext
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