Development and characterization of transgenic dominant male sterile rice toward an outcross-based breeding system

Genomic selection is attracting attention in the field of crop breeding. To apply genomic selection effectively for autogamous (self-pollinating) crops, an efficient outcross system is desired. Since dominant male sterility is a powerful tool for easy and successive outcross of autogamous crops, we...

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Veröffentlicht in:Breeding science. - 1998. - 68(2018), 2 vom: 01. März, Seite 248-257
1. Verfasser: Abe, Kiyomi (VerfasserIn)
Weitere Verfasser: Oshima, Masao, Akasaka, Maiko, Konagaya, Ken-Ichi, Nanasato, Yoshihiko, Okuzaki, Ayako, Taniguchi, Yojiro, Tanaka, Junichi, Tabei, Yutaka
Format: Online-Aufsatz
Sprache:English
Veröffentlicht: 2018
Zugriff auf das übergeordnete Werk:Breeding science
Schlagworte:Journal Article barnase flowering habits genomic selection male sterility null segregants outcross rice (Oryza sativa L.)
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520 |a Genomic selection is attracting attention in the field of crop breeding. To apply genomic selection effectively for autogamous (self-pollinating) crops, an efficient outcross system is desired. Since dominant male sterility is a powerful tool for easy and successive outcross of autogamous crops, we developed transgenic dominant male sterile rice (Oryza sativa L.) using the barnase gene that is expressed by the tapetum-specific promoter BoA9. Barnase-induced male sterile rice No. 10 (BMS10) was selected for its stable male sterility and normal growth characteristics. The BMS10 flowering habits, including heading date, flowering date, and daily flowering time of BMS10 tended to be delayed compared to wild type. When BMS10 and wild type were placed side-by-side and crossed under an open-pollinating condition, the seed-setting rate was <1.5%. When the clipping method was used to avoid the influence of late flowering habits, the seed-setting rate of BMS10 increased to a maximum of 86.4%. Although flowering synchronicity should be improved to increase the seed-setting rate, our results showed that this system can produce stable transgenic male sterility with normal female fertility in rice. The transgenic male sterile rice would promote a genomic selection-based breeding system in rice 
650 4 |a Journal Article 
650 4 |a barnase 
650 4 |a flowering habits 
650 4 |a genomic selection 
650 4 |a male sterility 
650 4 |a null segregants 
650 4 |a outcross 
650 4 |a rice (Oryza sativa L.) 
700 1 |a Oshima, Masao  |e verfasserin  |4 aut 
700 1 |a Akasaka, Maiko  |e verfasserin  |4 aut 
700 1 |a Konagaya, Ken-Ichi  |e verfasserin  |4 aut 
700 1 |a Nanasato, Yoshihiko  |e verfasserin  |4 aut 
700 1 |a Okuzaki, Ayako  |e verfasserin  |4 aut 
700 1 |a Taniguchi, Yojiro  |e verfasserin  |4 aut 
700 1 |a Tanaka, Junichi  |e verfasserin  |4 aut 
700 1 |a Tabei, Yutaka  |e verfasserin  |4 aut 
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773 1 8 |g volume:68  |g year:2018  |g number:2  |g day:01  |g month:03  |g pages:248-257 
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