Genetic dissection of pre-harvest sprouting resistance in an upland rice cultivar

Seed dormancy is important in rice breeding because it confers resistance to pre-harvest sprouting (PHS). To detect quantitative trait loci (QTLs) for pre-harvest sprouting resistance, we used chromosome segment substitution lines (CSSLs) derived from a cross between the Japanese upland rice cultiva...

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Veröffentlicht in:Breeding science. - 1998. - 68(2018), 2 vom: 01. März, Seite 200-209
1. Verfasser: Mizuno, Yosuke (VerfasserIn)
Weitere Verfasser: Yamanouchi, Utako, Hoshino, Tomoki, Nonoue, Yasunori, Nagata, Kazufumi, Fukuoka, Shuichi, Ando, Tsuyu, Yano, Masahiro, Sugimoto, Kazuhiko
Format: Online-Aufsatz
Sprache:English
Veröffentlicht: 2018
Zugriff auf das übergeordnete Werk:Breeding science
Schlagworte:Journal Article PHS resistance QTL germination pre-harvest sprouting resistance seed dormancy upland rice
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520 |a Seed dormancy is important in rice breeding because it confers resistance to pre-harvest sprouting (PHS). To detect quantitative trait loci (QTLs) for pre-harvest sprouting resistance, we used chromosome segment substitution lines (CSSLs) derived from a cross between the Japanese upland rice cultivar 'Owarihatamochi' and the lowland rice cultivar 'Koshihikari'. In the CSSLs, several chromosomal regions were associated with PHS resistance. Among these, the chromosome 9 segment from 'Owarihatamochi' had the greatest association with increased PHS resistance. Further QTL analysis using an advanced backcross population (BC4F2) derived from a 'Koshihikari' × 'Owarihatamochi' cross revealed two putative QTLs, here designated qSDR9.1 (Seed dormancy 9.1) and qSDR9.2, on chromosome 9. The 'Owarihatamochi' alleles of the two QTLs reduced germination. Further fine mapping revealed that qSDR9.1 and qSDR9.2 were located within 4.1-Mb and 2.3-Mb intervals (based on the 'Nipponbare' reference genome sequence) defined by the simple sequence repeat marker loci RM24039 and RM24260 and Indel_2 and RM24540, respectively. We thus identified two QTLs for PHS resistance in 'Owarihatamochi', even though resistance levels are relatively low in this cultivar. This unexpected finding suggests the advantages of using CSSLs for QTL detection 
650 4 |a Journal Article 
650 4 |a PHS resistance 
650 4 |a QTL 
650 4 |a germination 
650 4 |a pre-harvest sprouting resistance 
650 4 |a seed dormancy 
650 4 |a upland rice 
700 1 |a Yamanouchi, Utako  |e verfasserin  |4 aut 
700 1 |a Hoshino, Tomoki  |e verfasserin  |4 aut 
700 1 |a Nonoue, Yasunori  |e verfasserin  |4 aut 
700 1 |a Nagata, Kazufumi  |e verfasserin  |4 aut 
700 1 |a Fukuoka, Shuichi  |e verfasserin  |4 aut 
700 1 |a Ando, Tsuyu  |e verfasserin  |4 aut 
700 1 |a Yano, Masahiro  |e verfasserin  |4 aut 
700 1 |a Sugimoto, Kazuhiko  |e verfasserin  |4 aut 
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773 1 8 |g volume:68  |g year:2018  |g number:2  |g day:01  |g month:03  |g pages:200-209 
856 4 0 |u http://dx.doi.org/10.1270/jsbbs.17062  |3 Volltext 
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