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231225s2018 xx |||||o 00| ||eng c |
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|a 10.1111/jfd.12808
|2 doi
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|a eng
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| 100 |
1 |
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|a Wang, H
|e verfasserin
|4 aut
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| 245 |
1 |
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|a Rapid visual detection of cyprinid herpesvirus 2 by recombinase polymerase amplification combined with a lateral flow dipstick
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|c 2018
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|a Text
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|a ƒaComputermedien
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|a ƒa Online-Ressource
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|a Date Revised 27.02.2024
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|a published: Print-Electronic
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|a Citation Status Publisher
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|a © 2018 John Wiley & Sons Ltd.
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|a Herpesviral haematopoietic necrosis (HVHN), caused by cyprinid herpesvirus 2 (CyHV-2), causes significant losses in crucian carp (Carassius carassius) aquaculture. Rapid and convenient DNA assay detection of CyHV-2 is useful for field diagnosis. Recombinase polymerase amplification (RPA) is a novel isothermal DNA amplification and detection technology that can amplify DNA within 30 min at ~37°C by simulating in vivo DNA recombination. Herein, a rapid and convenient detection assay based on RPA with a lateral flow dipstick (LFD) was developed for detecting CyHV-2. The highly conserved ORF72 of CyHV-2 was targeted by specific and sensitive primers and probes. The optimized assay takes only 15 min at 38°C using a water bath, with analysis of products by 2% agarose gel electrophoresis within 30 min. A simple lateral flow strip based on the unique probe in reaction buffer was developed for visualization. The entire RPA-LFD assay takes 50 min less than the routine PCR method, is 100 times more sensitive and displays no cross-reaction with other aquatic viruses. The combined isothermal RPA and lateral flow assay (RPA-LFD) provides a simple, rapid, reliable method that could improve field diagnosis of CyHV-2 when resources are limited
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|a Journal Article
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|a CyHV-2
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4 |
|a crucian carp
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4 |
|a herpesviral haematopoietic necrosis
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| 650 |
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|a isothermal detection
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| 650 |
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|a lateral flow dipstick
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| 650 |
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4 |
|a recombinase polymerase amplification
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| 700 |
1 |
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|a Sun, M
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Xu, D
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Podok, P
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Xie, J
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Jiang, Ys
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Lu, Lq
|e verfasserin
|4 aut
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|i Enthalten in
|t Journal of fish diseases
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|u http://dx.doi.org/10.1111/jfd.12808
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