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231225s2018 xx |||||o 00| ||eng c |
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|a 10.1021/acs.langmuir.8b01248
|2 doi
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|a pubmed24n0947.xml
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|a DE-627
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|e rakwb
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|a eng
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|a Huang, Chi
|e verfasserin
|4 aut
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|a Redefining Molecular Amphipathicity in Reversing the "Coffee-Ring Effect"
|b Implications for Single Base Mutation Detection
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|c 2018
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
|b c
|2 rdamedia
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|a ƒa Online-Ressource
|b cr
|2 rdacarrier
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|a Date Completed 29.03.2019
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|a Date Revised 29.03.2019
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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|a The "coffee ring effect" is a natural phenomenon wherein sessile drops leave ring-shaped structures on the solid surfaces upon drying. It drives a nonuniform deposition of suspended compounds on the substrates, which adversely affects many processes, including surface-assisted biosensing and molecular self-assembly. In this study, we describe how the coffee ring effect can be eliminated by controlling the amphipathicity of the suspended compounds, for example, DNA modified with hydrophobic dye. Specifically, nuclease digestion of the hydrophilic DNA end converts the dye-labeled molecule into an amphipathic molecule (one with comparably weighted hydrophobic and hydrophilic ends) and reverses the coffee ring effect and results in a uniform disk-shaped feature deposition of the dye. The amphipathic product decreases the surface tension of the sessile drops and induces the Marangoni flow, which drives the uniform distribution of the amphipathic dye-labeled product in the drops. As a proof of concept, this strategy was used in a novel enzymatic amplification method for biosensing to eliminate the coffee ring effect on a nitrocellulose membrane and increase assay reliability and sensitivity. Importantly, the reported strategy for eliminating the coffee ring effect can be extended to other sessile drop systems for potentially improving assay reliability and sensitivity
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|a Journal Article
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|a Research Support, Non-U.S. Gov't
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|a Coloring Agents
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|a Collodion
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|a 9004-70-0
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|a DNA
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|a 9007-49-2
|2 NLM
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|a Wang, Jie
|e verfasserin
|4 aut
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|a Lv, Xiaobo
|e verfasserin
|4 aut
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|a Liu, Liu
|e verfasserin
|4 aut
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|a Liang, Ling
|e verfasserin
|4 aut
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|a Hu, Wei
|e verfasserin
|4 aut
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|a Luo, Changliang
|e verfasserin
|4 aut
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|a Wang, Fubing
|e verfasserin
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|a Yuan, Quan
|e verfasserin
|4 aut
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| 773 |
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|i Enthalten in
|t Langmuir : the ACS journal of surfaces and colloids
|d 1992
|g 34(2018), 23 vom: 12. Juni, Seite 6777-6783
|w (DE-627)NLM098181009
|x 1520-5827
|7 nnns
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| 773 |
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|g volume:34
|g year:2018
|g number:23
|g day:12
|g month:06
|g pages:6777-6783
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|u http://dx.doi.org/10.1021/acs.langmuir.8b01248
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