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231225s2018 xx |||||o 00| ||eng c |
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|a 10.1021/acs.langmuir.8b00976
|2 doi
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|a pubmed25n0946.xml
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|a (DE-627)NLM283892617
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|a (NLM)29742352
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|a DE-627
|b ger
|c DE-627
|e rakwb
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|a eng
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|a Periyasamy, Karthik
|e verfasserin
|4 aut
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|a Bioconversion of Lignocellulosic Biomass to Fermentable Sugars by Immobilized Magnetic Cellulolytic Enzyme Cocktails
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|c 2018
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
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|2 rdamedia
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|a ƒa Online-Ressource
|b cr
|2 rdacarrier
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|a Date Completed 01.04.2019
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|a Date Revised 01.04.2019
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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|a Enzyme cocktails of reusable, highly stable cellulolytic enzymes play an inevitable role in bioconversion of biomass to biofuels economically. Cellulase, xylanase and β-1,3-glucanase bound silica-amine functionalized iron oxide magnetic nanoparticles (ISN-CLEAs) were prepared and used as the biocatalyst for the depolymerization of cellulosic biomass into monomeric sugar in the present study. The Fe3O4-NPs and Fe3O4SiO2-NH2-NPs and ISN-CLEAs had an average hydrodynamic size of 82.2, 86.4, and 976.9 nm, respectively, which was confirmed by dynamic light scattering (DLS). About 97% of protein binding was achieved with 135 mM glutaraldehyde at 10 h of cross-linking time and successful binding was confirmed by Fourier transform infrared spectroscopy (FTIR). The ISN-CLEAs exhibited the highest thermal stability of 95% at 50 °C for 2 h and retained extended storage stability of 97% compared to 60% of its free counterpart. Besides, cross-linking allowed ISN-CLEAs reuse for at least eight consecutive cycles retaining over 70% of its initial activity. ISN-CLEAs exhibited approximately 15% increase in carbohydrate digestibility on sugar cane bagasse and eucalyptus pulp than the free enzyme
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|a Journal Article
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|a Research Support, Non-U.S. Gov't
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|a Biofuels
|2 NLM
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|a Cross-Linking Reagents
|2 NLM
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|a Enzymes, Immobilized
|2 NLM
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|a Sugars
|2 NLM
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|a lignocellulose
|2 NLM
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|a 11132-73-3
|2 NLM
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|a Silicon Dioxide
|2 NLM
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|a 7631-86-9
|2 NLM
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|a Lignin
|2 NLM
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|a 9005-53-2
|2 NLM
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| 700 |
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|a Santhalembi, Laishram
|e verfasserin
|4 aut
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|a Mortha, Gérard
|e verfasserin
|4 aut
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|a Aurousseau, Marc
|e verfasserin
|4 aut
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|a Boyer, Agnès
|e verfasserin
|4 aut
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|a Subramanian, Sivanesan
|e verfasserin
|4 aut
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| 773 |
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|i Enthalten in
|t Langmuir : the ACS journal of surfaces and colloids
|d 1985
|g 34(2018), 22 vom: 05. Juni, Seite 6546-6555
|w (DE-627)NLM098181009
|x 1520-5827
|7 nnns
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| 773 |
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|g volume:34
|g year:2018
|g number:22
|g day:05
|g month:06
|g pages:6546-6555
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|u http://dx.doi.org/10.1021/acs.langmuir.8b00976
|3 Volltext
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|d 34
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|e 22
|b 05
|c 06
|h 6546-6555
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