Isolation and characterization of novel bacterial strain present in a lab scale hybrid UASB reactor treating distillery spent wash

Recalcitrant compounds degrading novel bacteria was isolated from lab scale hybrid UASB reactor treating distillery spent wash, enriched with biomass activity. The granules were subjected to SEM analysis to classify and isolate the bacterial strains. The strain was ubiquitous, mesophilic, gram-negat...

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Veröffentlicht in:Environmental technology. - 1993. - 40(2019), 25 vom: 01. Nov., Seite 3351-3357
1. Verfasser: Thiyagu, R (VerfasserIn)
Weitere Verfasser: Sivarajan, P
Format: Online-Aufsatz
Sprache:English
Veröffentlicht: 2019
Zugriff auf das übergeordnete Werk:Environmental technology
Schlagworte:Journal Article 16S rRNA gene sequence distillery spent wash hybrid UASB reactor recalcitrant compounds RNA, Ribosomal, 16S Sewage
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520 |a Recalcitrant compounds degrading novel bacteria was isolated from lab scale hybrid UASB reactor treating distillery spent wash, enriched with biomass activity. The granules were subjected to SEM analysis to classify and isolate the bacterial strains. The strain was ubiquitous, mesophilic, gram-negative, motile, non-spore forming and cultivable optimally at 30°C at pH 7. Most potential isolates from the strains were subjected to 16S rRNA sequencing and branded as a member of diverse genera, gamma-proteobacteria, Stenotrophomonas sp. Based on 16S rRNA sequencing, genomic DNA extraction, PCR amplification, and phylogeny analysis the gram-negative bacteria was identified as Stenotrophomonas maltophilia and found to degrade distillery spent wash. The hybrid UASB reactor was operated for 360 days with 24 h HRT and has an optimum COD removal efficiency of 83.87% at an organic loading rates (OLRs) ranging within 0.25-27.40 kg COD/m3 d. Abbreviations: 16S rRNA: 16S ribosomal RNA; COD: Chemical oxygen demand; DNA: Deoxyribonucleic acid; dS/m: deciSiemens per metre; g/L: gram per litre; HRT: Hydraulic retention time; mg/L: milligram per litre; OLR: Organic loading rate; PCR: Polymerase chain reaction; RNA: Ribonucleic acid; UASB: Upflow anaerobic sludge blanket; VFA: Volatile fatty acid; VSS: Volatile suspended solid 
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