The novel cyst nematode effector protein 30D08 targets host nuclear functions to alter gene expression in feeding sites

Bibliographische Detailangaben
Veröffentlicht in:	The New phytologist. - 1979. - 219(2018), 2 vom: 06. Juli, Seite 697-713
1. Verfasser:	Verma, Anju (VerfasserIn)
Weitere Verfasser:	Lee, Chris, Morriss, Stephanie, Odu, Fiona, Kenning, Charlotte, Rizzo, Nancy, Spollen, William G, Lin, Marriam, McRae, Amanda G, Givan, Scott A, Hewezi, Tarek, Hussey, Richard, Davis, Eric L, Baum, Thomas J, Mitchum, Melissa G
Format:	Online-Aufsatz
Sprache:	English
Veröffentlicht:	2018
Zugriff auf das übergeordnete Werk:	The New phytologist
Schlagworte:	Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Heterodera SMU2 alternative splicing cyst nematode effector nucleus spliceosome mehr... syncytium Helminth Proteins Nuclear Localization Signals

Beschreibung
Zusammenfassung:	© 2018 The Authors. New Phytologist © 2018 New Phytologist Trust. Cyst nematodes deliver effector proteins into host cells to manipulate cellular processes and establish a metabolically hyperactive feeding site. The novel 30D08 effector protein is produced in the dorsal gland of parasitic juveniles, but its function has remained unknown. We demonstrate that expression of 30D08 contributes to nematode parasitism, the protein is packaged into secretory granules and it is targeted to the plant nucleus where it interacts with SMU2 (homolog of suppressor of mec-8 and unc-52 2), an auxiliary spliceosomal protein. We show that SMU2 is expressed in feeding sites and an smu2 mutant is less susceptible to nematode infection. In Arabidopsis expressing 30D08 under the SMU2 promoter, several genes were found to be alternatively spliced and the most abundant functional classes represented among differentially expressed genes were involved in RNA processing, transcription and binding, as well as in development, and hormone and secondary metabolism, representing key cellular processes known to be important for feeding site formation. In conclusion, we demonstrated that the 30D08 effector is secreted from the nematode and targeted to the plant nucleus where its interaction with a host auxiliary spliceosomal protein may alter the pre-mRNA splicing and expression of a subset of genes important for feeding site formation
Beschreibung:	Date Completed 20.09.2019 Date Revised 30.09.2020 published: Print-Electronic GENBANK: MF959551 Citation Status MEDLINE
ISSN:	1469-8137
DOI:	10.1111/nph.15179