Downregulating aspen xylan biosynthetic GT43 genes in developing wood stimulates growth via reprograming of the transcriptome

Downregulating aspen xylan biosynthetic GT43 genes in developing wood stimulates growth via reprograming of the transcriptome

© 2018 The Authors. New Phytologist © 2018 New Phytologist Trust.

Bibliographische Detailangaben
Veröffentlicht in:The New phytologist. - 1984. - 219(2018), 1 vom: 15. Juli, Seite 230-245
1. Verfasser: Ratke, Christine (VerfasserIn)
Weitere Verfasser: Terebieniec, Barbara K, Winestrand, Sandra, Derba-Maceluch, Marta, Grahn, Thomas, Schiffthaler, Bastian, Ulvcrona, Thomas, Özparpucu, Merve, Rüggeberg, Markus, Lundqvist, Sven-Olof, Street, Nathaniel R, Jönsson, Leif J, Mellerowicz, Ewa J
Format: Online-Aufsatz
Sprache:English
Veröffentlicht: 2018
Zugriff auf das übergeordnete Werk:The New phytologist
Schlagworte:Journal Article Research Support, Non-U.S. Gov't Populus GT43 cellulose microfibril angle saccharification secondary wall wood development xylan biosynthesis Plant Proteins mehr... Sugars Xylans Cellulose 9004-34-6 Lignin 9005-53-2
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100 1 |a Ratke, Christine  |e verfasserin  |4 aut 
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520 |a Xylan is one of the main compounds determining wood properties in hardwood species. The xylan backbone is thought to be synthesized by a synthase complex comprising two members of the GT43 family. We downregulated all GT43 genes in hybrid aspen (Populus tremula × tremuloides) to understand their involvement in xylan biosynthesis. All three clades of the GT43 family were targeted for downregulation using RNA interference individually or in different combinations, either constitutively or specifically in developing wood. Simultaneous downregulation in developing wood of the B (IRX9) and C (IRX14) clades resulted in reduced xylan Xyl content relative to reducing end sequence, supporting their role in xylan backbone biosynthesis. This was accompanied by a higher lignocellulose saccharification efficiency. Unexpectedly, GT43 suppression in developing wood led to an overall growth stimulation, xylem cell wall thinning and a shift in cellulose orientation. Transcriptome profiling of these transgenic lines indicated that cell cycling was stimulated and secondary wall biosynthesis was repressed. We suggest that the reduced xylan elongation is sensed by the cell wall integrity surveying mechanism in developing wood. Our results show that wood-specific suppression of xylan-biosynthetic GT43 genes activates signaling responses, leading to increased growth and improved lignocellulose saccharification 
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650 4 |a cellulose microfibril angle 
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650 4 |a secondary wall 
650 4 |a wood development 
650 4 |a xylan biosynthesis 
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700 1 |a Terebieniec, Barbara K  |e verfasserin  |4 aut 
700 1 |a Winestrand, Sandra  |e verfasserin  |4 aut 
700 1 |a Derba-Maceluch, Marta  |e verfasserin  |4 aut 
700 1 |a Grahn, Thomas  |e verfasserin  |4 aut 
700 1 |a Schiffthaler, Bastian  |e verfasserin  |4 aut 
700 1 |a Ulvcrona, Thomas  |e verfasserin  |4 aut 
700 1 |a Özparpucu, Merve  |e verfasserin  |4 aut 
700 1 |a Rüggeberg, Markus  |e verfasserin  |4 aut 
700 1 |a Lundqvist, Sven-Olof  |e verfasserin  |4 aut 
700 1 |a Street, Nathaniel R  |e verfasserin  |4 aut 
700 1 |a Jönsson, Leif J  |e verfasserin  |4 aut 
700 1 |a Mellerowicz, Ewa J  |e verfasserin  |4 aut 
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773 1 8 |g volume:219  |g year:2018  |g number:1  |g day:15  |g month:07  |g pages:230-245 
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