Colorimetric method for determining viability of sea urchin sperm applied in toxicity tests
The aim of this study was to improve the methodological procedure for the evaluation of sea urchin (Lytechinus variegatus) sperm sensitivity in MTT (3-(4,5-dimethylthiazol-2yl)-2,5 diphenyltetrazolium bromide) enzyme reduction assays with the formation of formazan (purple color) in the interior of v...
Ausführliche Beschreibung
Bibliographische Detailangaben
| Veröffentlicht in: | Ecotoxicology (London, England). - 1992. - 27(2018), 5 vom: 24. Juli, Seite 499-504
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| 1. Verfasser: |
Resgalla, Charrid Jr
(VerfasserIn) |
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| Weitere Verfasser: |
Máximo, Marcus Vinícius,
Brasil, Mirella do Nascimento,
Pessatti, Marcos Luiz |
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| Format: | Online-Aufsatz
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| Sprache: | English |
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| Veröffentlicht: |
2018
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| Zugriff auf das übergeordnete Werk: | Ecotoxicology (London, England)
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| Schlagworte: | Journal Article
Calcium channel
Cytotoxicity assay
Lytechinus variegatus
Metabolic activation
Sensitivity
Verapamil
Ammonium Compounds
Formazans
Metals, Heavy
mehr...
Tetrazolium Salts
Thiazoles
Water Pollutants, Chemical
Sodium Dodecyl Sulfate
368GB5141J
thiazolyl blue
EUY85H477I |
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