Two ancestral genes shaped the Xanthomonas campestris TAL effector gene repertoire

Two ancestral genes shaped the Xanthomonas campestris TAL effector gene repertoire

© 2018 The Authors New Phytologist © 2018 New Phytologist Trust.

Bibliographische Detailangaben
Veröffentlicht in:The New phytologist. - 1979. - 219(2018), 1 vom: 04. Juli, Seite 391-407
1. Verfasser: Denancé, Nicolas (VerfasserIn)
Weitere Verfasser: Szurek, Boris, Doyle, Erin L, Lauber, Emmanuelle, Fontaine-Bodin, Lisa, Carrère, Sébastien, Guy, Endrick, Hajri, Ahmed, Cerutti, Aude, Boureau, Tristan, Poussier, Stéphane, Arlat, Matthieu, Bogdanove, Adam J, Noël, Laurent D
Format: Online-Aufsatz
Sprache:English
Veröffentlicht: 2018
Zugriff auf das übergeordnete Werk:The New phytologist
Schlagworte:Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Brassica rapa Brassicaceae Hax TALE Xanthomonas campestris black rot Transcription Activator-Like Effectors
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245 1 0 |a Two ancestral genes shaped the Xanthomonas campestris TAL effector gene repertoire 
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520 |a © 2018 The Authors New Phytologist © 2018 New Phytologist Trust. 
520 |a Xanthomonas transcription activator-like effectors (TALEs) are injected inside plant cells to promote host susceptibility by enhancing transcription of host susceptibility genes. TALE-encoding (tal) genes were thought to be absent from Brassicaceae-infecting Xanthomonas campestris (Xc) genomes based on four reference genomic sequences. We discovered tal genes in 26 of 49 Xc strains isolated worldwide and used a combination of single molecule real time (SMRT) and tal amplicon sequencing to yield a near-complete description of the TALEs found in Xc (Xc TALome). The 53 sequenced tal genes encode 21 distinct DNA binding domains that sort into seven major DNA binding specificities. In silico analysis of the Brassica rapa promoterome identified a repertoire of predicted TALE targets, five of which were experimentally validated using quantitative reverse transcription polymerase chain reaction. The Xc TALome shows multiple signs of DNA rearrangements that probably drove its evolution from two ancestral tal genes. We discovered that Tal12a and Tal15a of Xcc strain Xca5 contribute together in the development of disease symptoms on susceptible B. oleracea var. botrytis cv Clovis. This large and polymorphic repertoire of TALEs opens novel perspectives for elucidating TALE-mediated susceptibility of Brassicaceae to black rot disease and for understanding the molecular processes underlying TALE evolution 
650 4 |a Journal Article 
650 4 |a Research Support, Non-U.S. Gov't 
650 4 |a Research Support, U.S. Gov't, Non-P.H.S. 
650 4 |a Brassica rapa 
650 4 |a Brassicaceae 
650 4 |a Hax 
650 4 |a TALE 
650 4 |a Xanthomonas campestris 
650 4 |a black rot 
650 7 |a Transcription Activator-Like Effectors  |2 NLM 
700 1 |a Szurek, Boris  |e verfasserin  |4 aut 
700 1 |a Doyle, Erin L  |e verfasserin  |4 aut 
700 1 |a Lauber, Emmanuelle  |e verfasserin  |4 aut 
700 1 |a Fontaine-Bodin, Lisa  |e verfasserin  |4 aut 
700 1 |a Carrère, Sébastien  |e verfasserin  |4 aut 
700 1 |a Guy, Endrick  |e verfasserin  |4 aut 
700 1 |a Hajri, Ahmed  |e verfasserin  |4 aut 
700 1 |a Cerutti, Aude  |e verfasserin  |4 aut 
700 1 |a Boureau, Tristan  |e verfasserin  |4 aut 
700 1 |a Poussier, Stéphane  |e verfasserin  |4 aut 
700 1 |a Arlat, Matthieu  |e verfasserin  |4 aut 
700 1 |a Bogdanove, Adam J  |e verfasserin  |4 aut 
700 1 |a Noël, Laurent D  |e verfasserin  |4 aut 
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773 1 8 |g volume:219  |g year:2018  |g number:1  |g day:04  |g month:07  |g pages:391-407 
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