RNA interference of GhPEPC2 enhanced seed oil accumulation and salt tolerance in Upland cotton

RNA interference of GhPEPC2 enhanced seed oil accumulation and salt tolerance in Upland cotton

Copyright © 2018 Elsevier B.V. All rights reserved.

Bibliographische Detailangaben
Veröffentlicht in:Plant science : an international journal of experimental plant biology. - 1985. - 271(2018) vom: 17. Juni, Seite 52-61
1. Verfasser: Zhao, Yanpeng (VerfasserIn)
Weitere Verfasser: Huang, Yi, Wang, Yumei, Cui, Yupeng, Liu, Zhengjie, Hua, Jinping
Format: Online-Aufsatz
Sprache:English
Veröffentlicht: 2018
Zugriff auf das übergeordnete Werk:Plant science : an international journal of experimental plant biology
Schlagworte:Journal Article GhPEPC2 Oil content RNA interference RNA-Seq Salt stress Upland cotton Plant Oils Phosphoenolpyruvate Carboxykinase (ATP) EC 4.1.1.49
Beschreibung
Zusammenfassung:Copyright © 2018 Elsevier B.V. All rights reserved.
Phosphoenolpyruvate carboxylase (PEPCase) mainly produces oxaloacetic acid for tricarboxylic acid (TCA) cycle. Here we reported that GhPEPC2 silencing with PEPC2-RNAi vector could regulate oil and protein accumulation in cottonseeds. In GhPEPC2 transgenic plants, PEPCase activities in immature embryos were significantly reduced, and the oil content in seed kernel was increased 7.3 percentages, whereas total proteins decreased 5.65 percentages. Compared to wild type, agronomical traits of transgenic plant were obviously unaffected. Furthermore, gene expression profile of GhPEPC2 transgenic seeds were investigated using RNA-seq, most lipid synthesis related genes were up-regulated, but amino acid metabolic related genes were down-regulated. In addition, the GhPEPC2 transgenic cotton seedlings were stressed using sodium salts at seedling stage, and the salt tolerance was significantly enhanced. Our observations of GhPEPC2 in cotton would shade light on understanding the regulation of oil content, protein accumulation and salt tolerance enhancement in other plants
Beschreibung:Date Completed 27.08.2018
Date Revised 30.09.2020
published: Print-Electronic
Citation Status MEDLINE
ISSN:1873-2259
DOI:10.1016/j.plantsci.2018.03.015