RNA interference of GhPEPC2 enhanced seed oil accumulation and salt tolerance in Upland cotton

Copyright © 2018 Elsevier B.V. All rights reserved.

Détails bibliographiques
Publié dans:Plant science : an international journal of experimental plant biology. - 1985. - 271(2018) vom: 17. Juni, Seite 52-61
Auteur principal: Zhao, Yanpeng (Auteur)
Autres auteurs: Huang, Yi, Wang, Yumei, Cui, Yupeng, Liu, Zhengjie, Hua, Jinping
Format: Article en ligne
Langue:English
Publié: 2018
Accès à la collection:Plant science : an international journal of experimental plant biology
Sujets:Journal Article GhPEPC2 Oil content RNA interference RNA-Seq Salt stress Upland cotton Plant Oils Phosphoenolpyruvate Carboxykinase (ATP) EC 4.1.1.49
Description
Résumé:Copyright © 2018 Elsevier B.V. All rights reserved.
Phosphoenolpyruvate carboxylase (PEPCase) mainly produces oxaloacetic acid for tricarboxylic acid (TCA) cycle. Here we reported that GhPEPC2 silencing with PEPC2-RNAi vector could regulate oil and protein accumulation in cottonseeds. In GhPEPC2 transgenic plants, PEPCase activities in immature embryos were significantly reduced, and the oil content in seed kernel was increased 7.3 percentages, whereas total proteins decreased 5.65 percentages. Compared to wild type, agronomical traits of transgenic plant were obviously unaffected. Furthermore, gene expression profile of GhPEPC2 transgenic seeds were investigated using RNA-seq, most lipid synthesis related genes were up-regulated, but amino acid metabolic related genes were down-regulated. In addition, the GhPEPC2 transgenic cotton seedlings were stressed using sodium salts at seedling stage, and the salt tolerance was significantly enhanced. Our observations of GhPEPC2 in cotton would shade light on understanding the regulation of oil content, protein accumulation and salt tolerance enhancement in other plants
Description:Date Completed 27.08.2018
Date Revised 30.09.2020
published: Print-Electronic
Citation Status MEDLINE
ISSN:1873-2259
DOI:10.1016/j.plantsci.2018.03.015