Increasing genetic variability in oilseed rape (Brassica napus) - Genotypes and phenotypes of oilseed rape transformed by wild type Agrobacterium rhizogenes

Copyright © 2018 Elsevier B.V. All rights reserved.

Bibliographische Detailangaben
Veröffentlicht in:Plant science : an international journal of experimental plant biology. - 1985. - 271(2018) vom: 17. Juni, Seite 20-26
1. Verfasser: Hegelund, Josefine Nymark (VerfasserIn)
Weitere Verfasser: Liang, Chang, Lauridsen, Uffe Bjerre, Kemp, Oliver, Lütken, Henrik, Müller, Renate
Format: Online-Aufsatz
Sprache:English
Veröffentlicht: 2018
Zugriff auf das übergeordnete Werk:Plant science : an international journal of experimental plant biology
Schlagworte:Journal Article Agrobacterium rhizogenes Brassica napus Natural transformation Oil quality Oilseed rape Ri-phenotype
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245 1 0 |a Increasing genetic variability in oilseed rape (Brassica napus) - Genotypes and phenotypes of oilseed rape transformed by wild type Agrobacterium rhizogenes 
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500 |a Citation Status MEDLINE 
520 |a Copyright © 2018 Elsevier B.V. All rights reserved. 
520 |a Brassica napus (oilseed rape) is a major oil crop worldwide. Due to the short domestication period of oilseed rape the genetic variability is limited compared to other crops. Transfer of rol and aux genes from Agrobacterium rhizogenes is used in horticulture to increase genetic variability. In the current study, we explore transformation by A. rhizogenes as a biotechnological approach in breeding for more branched and shorter oilseed rape. In the 2nd generation of transformed oilseed rape, branch numbers increased significantly by 49% from 7.7 ± 0.4 to 11.5 ± 1.9 when comparing rol+/aux+ plants with WT. Simultaneously, the apical height of plants was reduced by 25% from 81.3 ± 1.9 cm to 62.4 ± 6.7 cm in rol+/aux+ plants at the onset of flowering. Reproductive parameters affecting yield as seed size and number were negatively affected in rol+/aux+ plants. Interestingly, oil composition was changed in rol+/aux+ seeds. Oleic acid (ω9) contents were reduced by more than 3% whereas α-linolenic acid (ω6) increased by more than 25% in mature seeds. To obtain shorter and more branched breeding material of oilseed rape we suggest crossing plants with the rol+/aux+ genotype back into the parental breeding line. This could reduce the negative impact of rol+/aux+ on yield 
650 4 |a Journal Article 
650 4 |a Agrobacterium rhizogenes 
650 4 |a Brassica napus 
650 4 |a Natural transformation 
650 4 |a Oil quality 
650 4 |a Oilseed rape 
650 4 |a Ri-phenotype 
700 1 |a Liang, Chang  |e verfasserin  |4 aut 
700 1 |a Lauridsen, Uffe Bjerre  |e verfasserin  |4 aut 
700 1 |a Kemp, Oliver  |e verfasserin  |4 aut 
700 1 |a Lütken, Henrik  |e verfasserin  |4 aut 
700 1 |a Müller, Renate  |e verfasserin  |4 aut 
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773 1 8 |g volume:271  |g year:2018  |g day:17  |g month:06  |g pages:20-26 
856 4 0 |u http://dx.doi.org/10.1016/j.plantsci.2018.03.003  |3 Volltext 
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