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231225s2018 xx |||||o 00| ||eng c |
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|a 10.1016/j.plaphy.2018.03.032
|2 doi
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|a pubmed25n0942.xml
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|a (DE-627)NLM282757422
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|a (NLM)29626705
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|a (PII)S0981-9428(18)30155-4
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|a DE-627
|b ger
|c DE-627
|e rakwb
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|a eng
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1 |
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|a Kageyama, Hakuto
|e verfasserin
|4 aut
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|a Biosynthetic pathways of glycinebetaine in Thalassiosira pseudonana; functional characterization of enzyme catalyzing three-step methylation of glycine
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|c 2018
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
|b c
|2 rdamedia
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|a ƒa Online-Ressource
|b cr
|2 rdacarrier
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|a Date Completed 26.07.2018
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|a Date Revised 30.09.2020
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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|a Copyright © 2018 Elsevier Masson SAS. All rights reserved.
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|a Betaine (trimethylglycine) is an important compatible solute that accumulates in response to abiotic stresses such as drought and salinity. Biosynthetic pathways of betaine have been extensively studied, but it remains to be clarified on algae. A diatom Thalassiosira pseudonana CCMP1335 is an important component of marine ecosystems. Here we show that the genome sequence of Thalassiosira suggests the presence of two biosynthetic pathways for betaine, via three step methylation of glycine and via two step oxidation of choline. The choline oxidation via choline dehydrogenase was suggested and its sequential characteristics were analyzed. A candidate gene TpORF1 for glycine methylation encodes a protein consisted of 574 amino acids with two putative tandem repeat methyltransferase domains. The TpORF1 was expressed in E. coli, and the purified protein was shown to synthesize betaine via three step methylation of glycine and designated as TpGSDMT. The proteins containing C-terminal half or N-terminal half were expressed in E. coli and exhibited the methyl transferase activities with different substrate specificity for glycine, sarcosine and dimethylglycine. Upregulation of TpGSDMT transcription and betaine levels were observed at high salinity, suggesting the importance of TpGSDMT for salt tolerance in T. pseudonana cells
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|a Journal Article
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|a Betaine
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|a Betaine synthetic pathway
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|a Choline dehydrogenase
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|a GSDMT
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|a Thalassiosira pseudonana
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|a Betaine
|2 NLM
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|a 3SCV180C9W
|2 NLM
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|a Methyltransferases
|2 NLM
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|a EC 2.1.1.-
|2 NLM
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|a Choline
|2 NLM
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|a N91BDP6H0X
|2 NLM
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| 650 |
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|a Glycine
|2 NLM
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|a TE7660XO1C
|2 NLM
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|a Tanaka, Yoshito
|e verfasserin
|4 aut
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|a Takabe, Teruhiro
|e verfasserin
|4 aut
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| 773 |
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|i Enthalten in
|t Plant physiology and biochemistry : PPB
|d 1991
|g 127(2018) vom: 10. Juni, Seite 248-255
|w (DE-627)NLM098178261
|x 1873-2690
|7 nnns
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|g volume:127
|g year:2018
|g day:10
|g month:06
|g pages:248-255
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|u http://dx.doi.org/10.1016/j.plaphy.2018.03.032
|3 Volltext
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