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231225s2018 xx |||||o 00| ||eng c |
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|a 10.1111/vcp.12602
|2 doi
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|a pubmed25n0941.xml
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|a (DE-627)NLM282339817
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|a (NLM)29578657
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|a DE-627
|b ger
|c DE-627
|e rakwb
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| 041 |
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|a eng
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| 100 |
1 |
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|a Cuq, Benoît
|e verfasserin
|4 aut
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| 245 |
1 |
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|a Reproducibility, stability, and biological variability of thrombin generation using calibrated automated thrombography in healthy dogs
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| 264 |
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|c 2018
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| 336 |
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
|b c
|2 rdamedia
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| 338 |
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|a ƒa Online-Ressource
|b cr
|2 rdacarrier
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| 500 |
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|a Date Completed 11.10.2018
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|a Date Revised 10.12.2019
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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|a © 2018 American Society for Veterinary Clinical Pathology.
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|a BACKGROUND: Thrombin plays a central role in hemostasis and thrombosis. Calibrated automated thrombography (CAT), a thrombin generation assay, may be a useful test for hemostatic disorders in dogs
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|a OBJECTIVES: To describe CAT results in a group of healthy dogs, and assess preanalytical variables and biological variability
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| 520 |
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|a ANIMALS: Forty healthy dogs were enrolled
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|a METHODS: Lag time (Lag), time to peak (ttpeak), peak thrombin generation (peak), and endogenous thrombin potential (ETP) were measured. Direct jugular venipuncture and winged-needle catheter-assisted saphenous venipuncture were used to collect samples from each dog, and results were compared between methods. Sample stability at -80°C was assessed over 12 months in a subset of samples. Biological variability of CAT was assessed via nested ANOVA using samples obtained weekly from a subset of 9 dogs for 4 consecutive weeks
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|a RESULTS: Samples for CAT were stable at -80°C over 12 months of storage. Samples collected via winged-needle catheter venipuncture showed poor repeatability compared to direct venipuncture samples; there was also poor agreement between the 2 sampling methods. Intra-individual variability of CAT parameters was below 25%; inter-individual variability ranged from 36.9% to 78.5%
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|a CONCLUSIONS: Measurement of thrombin generation using CAT appears to be repeatable in healthy dogs, and samples are stable for at least 12 months when stored at -80°C. Direct venipuncture sampling is recommended for CAT. Low indices of individuality suggest that subject-based reference intervals are more suitable when interpreting CAT results
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|a Evaluation Study
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|a Journal Article
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| 650 |
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4 |
|a Canine
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| 650 |
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4 |
|a hemostasis
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| 650 |
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7 |
|a Thrombin
|2 NLM
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| 650 |
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|a EC 3.4.21.5
|2 NLM
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| 700 |
1 |
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|a Blois, Shauna L
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Wood, R Darren
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Monteith, Gabrielle
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Abrams-Ogg, Anthony C
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Bédard, Christian
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Wood, Geoffrey A
|e verfasserin
|4 aut
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| 773 |
0 |
8 |
|i Enthalten in
|t Veterinary clinical pathology
|d 1975
|g 47(2018), 2 vom: 23. Juni, Seite 218-226
|w (DE-627)NLM098159984
|x 1939-165X
|7 nnas
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| 773 |
1 |
8 |
|g volume:47
|g year:2018
|g number:2
|g day:23
|g month:06
|g pages:218-226
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| 856 |
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|u http://dx.doi.org/10.1111/vcp.12602
|3 Volltext
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|d 47
|j 2018
|e 2
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|h 218-226
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