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231225s2018 xx |||||o 00| ||eng c |
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|a 10.1016/j.plantsci.2018.02.002
|2 doi
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|a pubmed25n0940.xml
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|a (NLM)29576065
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|a (PII)S0168-9452(17)30848-8
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|a DE-627
|b ger
|c DE-627
|e rakwb
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|a eng
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| 100 |
1 |
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|a Wu, Ting-Ying
|e verfasserin
|4 aut
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| 245 |
1 |
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|a Facilitated citrate-dependent iron translocation increases rice endosperm iron and zinc concentrations
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|c 2018
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
|b c
|2 rdamedia
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|a ƒa Online-Ressource
|b cr
|2 rdacarrier
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|a Date Completed 04.09.2018
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|a Date Revised 04.12.2021
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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|a Copyright © 2018 The Authors. Published by Elsevier B.V. All rights reserved.
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|a Iron deficiency affects one third of the world population. Most iron biofortification strategies have focused on genes involved in iron uptake and storage but facilitating internal long-distance iron translocation has been understudied for increasing grain iron concentrations. Citrate is a primary iron chelator, and the transporter FERRIC REDUCTASE DEFECTIVE 3 (FRD3) loads citrate into the xylem. We have expressed AtFRD3 in combination with AtNAS1 (NICOTIANAMINE SYNTHASE 1) and PvFER (FERRITIN) or with PvFER alone to facilitate long-distance iron transport together with efficient iron uptake and storage in the rice endosperm. The citrate and iron concentrations in the xylem sap of transgenic plants increased two-fold compared to control plants. Iron and zinc levels increased significantly in polished and unpolished rice grains to more than 70% of the recommended estimated average requirement (EAR) for iron and 140% of the recommended EAR for zinc in polished rice grains. Furthermore, the transformed lines showed normal phenotypic growth, were tolerant to iron deficiency and aluminum toxicity, and had grain cadmium levels similar to control plants. Together, our results demonstrate that deploying FRD for iron biofortification has no obvious anti-nutritive effects and should be considered as an effective strategy for reducing human iron deficiency anemia
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|a Journal Article
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|a AtFRD3
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|a Biofortification
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|a Iron
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|a Rice
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|a Zinc
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|a Arabidopsis Proteins
|2 NLM
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|a FRD3 protein, Arabidopsis
|2 NLM
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|a Membrane Transport Proteins
|2 NLM
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|a Plant Proteins
|2 NLM
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|a Cadmium
|2 NLM
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|a 00BH33GNGH
|2 NLM
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|a Citric Acid
|2 NLM
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|a 2968PHW8QP
|2 NLM
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|a Ferritins
|2 NLM
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|a 9007-73-2
|2 NLM
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|a Aluminum
|2 NLM
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|a CPD4NFA903
|2 NLM
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| 650 |
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|a Iron
|2 NLM
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|a E1UOL152H7
|2 NLM
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|a Alkyl and Aryl Transferases
|2 NLM
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|a EC 2.5.-
|2 NLM
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|a nicotianamine synthase
|2 NLM
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|a EC 2.5.-
|2 NLM
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|a Zinc
|2 NLM
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| 650 |
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|a J41CSQ7QDS
|2 NLM
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| 700 |
1 |
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|a Gruissem, Wilhelm
|e verfasserin
|4 aut
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| 700 |
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|a Bhullar, Navreet K
|e verfasserin
|4 aut
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| 773 |
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|i Enthalten in
|t Plant science : an international journal of experimental plant biology
|d 1985
|g 270(2018) vom: 10. Mai, Seite 13-22
|w (DE-627)NLM098174193
|x 1873-2259
|7 nnns
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| 773 |
1 |
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|g volume:270
|g year:2018
|g day:10
|g month:05
|g pages:13-22
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|u http://dx.doi.org/10.1016/j.plantsci.2018.02.002
|3 Volltext
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|d 270
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|h 13-22
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