Combined and breed-specific RIs for hematologic, biochemical, and hormonal analytes in Chios and Florina adult rams

© 2018 American Society for Veterinary Clinical Pathology.

Bibliographische Detailangaben
Veröffentlicht in:Veterinary clinical pathology. - 1975. - 47(2018), 1 vom: 25. März, Seite 56-68
1. Verfasser: Oikonomidis, Ioannis L (VerfasserIn)
Weitere Verfasser: Brozos, Christos, Kiossis, Evangelos, Kritsepi-Konstantinou, Maria
Format: Online-Aufsatz
Sprache:English
Veröffentlicht: 2018
Zugriff auf das übergeordnete Werk:Veterinary clinical pathology
Schlagworte:Journal Article Clinical biochemistry complete blood count endocrinology male sheep partitioning reference values Hormones
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245 1 0 |a Combined and breed-specific RIs for hematologic, biochemical, and hormonal analytes in Chios and Florina adult rams 
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500 |a Date Revised 10.09.2018 
500 |a published: Print 
500 |a Citation Status MEDLINE 
520 |a © 2018 American Society for Veterinary Clinical Pathology. 
520 |a BACKGROUND: Clinical pathology investigations are essential to evaluate the health status and practice management of ruminants. Appropriate and accurate RIs are required for the interpretation of laboratory results. Despite substantial phenotypic differences and management-related disparities between male and female sheep, RIs in rams are sparse and limited by small reference populations and outdated methods 
520 |a OBJECTIVES: The purpose of this study was to establish RIs for hematologic, biochemical, and hormonal analytes in adult rams using controlled preanalytic and analytic procedures, and to investigate whether breed-dependent partitioning of RIs is required 
520 |a METHODS: Reference individuals were selected by a direct a priori method. Blood was collected from 128 healthy adult rams representing 2 Greek breeds (64 Chios and 64 Florina). Hematologic analysis was performed on the Advia 120, while differential WBC counts were manually performed on Giemsa-stained blood smears. Flexor E, AVL 9180 Electrolyte Analyzer, and Immulite 1000 were used for biochemical and hormonal analyses, respectively. Combined and breed-specific RIs were calculated using nonparametric and robust methods, respectively 
520 |a RESULTS: Five outliers were detected based on laboratory results. Partitioning of RIs, based on both statistical and nonstatistical criteria, was recommended for some of the analytes 
520 |a CONCLUSIONS: The RIs are in some agreement with those in the literature. The observed differences may be attributed to preanalytic and analytic factors. The RIs determined in this study can be used as a guide for the interpretation of laboratory results in rams and can potentially be adopted by veterinary laboratories, provided that similar equipment and reagents are used 
650 4 |a Journal Article 
650 4 |a Clinical biochemistry 
650 4 |a complete blood count 
650 4 |a endocrinology 
650 4 |a male sheep 
650 4 |a partitioning 
650 4 |a reference values 
650 7 |a Hormones  |2 NLM 
700 1 |a Brozos, Christos  |e verfasserin  |4 aut 
700 1 |a Kiossis, Evangelos  |e verfasserin  |4 aut 
700 1 |a Kritsepi-Konstantinou, Maria  |e verfasserin  |4 aut 
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773 1 8 |g volume:47  |g year:2018  |g number:1  |g day:25  |g month:03  |g pages:56-68 
856 4 0 |u http://dx.doi.org/10.1111/vcp.12558  |3 Volltext 
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