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231225s2018 xx |||||o 00| ||eng c |
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|a 10.1016/j.plaphy.2018.03.014
|2 doi
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|a pubmed24n0940.xml
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|a (DE-627)NLM282103708
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|a (NLM)29554573
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|a (PII)S0981-9428(18)30129-3
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|a DE-627
|b ger
|c DE-627
|e rakwb
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|a eng
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1 |
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|a Fontanini, Debora
|e verfasserin
|4 aut
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|a The phytochelatin synthase from Nitella mucronata (Charophyta) plays a role in the homeostatic control of iron(II)/(III)
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|c 2018
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
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|2 rdamedia
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|a ƒa Online-Ressource
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|2 rdacarrier
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|a Date Completed 26.07.2018
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|a Date Revised 30.09.2020
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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|a Copyright © 2018 Elsevier Masson SAS. All rights reserved.
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|a Although some charophytes (sister group to land plants) have been shown to synthesize phytochelatins (PCs) in response to cadmium (Cd), the functional characterization of their phytochelatin synthase (PCS) is still completely lacking. To investigate the metal response and the presence of PCS in charophytes, we focused on the species Nitella mucronata. A 40 kDa immunoreactive PCS band was revealed in mono-dimensional western blot by using a polyclonal antibody against Arabidopsis thaliana PCS1. In two-dimensional western blot, the putative PCS showed various spots with acidic isoelectric points, presumably originated by post-translational modifications. Given the PCS constitutive expression in N. mucronata, we tested its possible involvement in the homeostasis of metallic micronutrients, using physiological concentrations of iron (Fe) and zinc (Zn), and verified its role in the detoxification of a non-essential metal, such as Cd. Neither in vivo nor in vitro exposure to Zn resulted in PCS activation and PC significant biosynthesis, while Fe(II)/(III) and Cd were able to activate the PCS in vitro, as well as to induce PC accumulation in vivo. While Cd toxicity was evident from electron microscopy observations, the normal morphology of cells and organelles following Fe treatments was preserved. The overall results support a function of PCS and PCs in managing Fe homeostasis in the carophyte N. mucronata
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|a Journal Article
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|a Cadmium
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|a Charophytes
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|a Iron
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|a Metals
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|a Phytochelatin synthase
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|a Phytochelatins
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|a Plant Proteins
|2 NLM
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|a Iron
|2 NLM
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|a E1UOL152H7
|2 NLM
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|a Aminoacyltransferases
|2 NLM
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|a EC 2.3.2.-
|2 NLM
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|a glutathione gamma-glutamylcysteinyltransferase
|2 NLM
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|a EC 2.3.2.15
|2 NLM
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|a Andreucci, Andrea
|e verfasserin
|4 aut
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|a Ruffini Castiglione, Monica
|e verfasserin
|4 aut
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|a Basile, Adriana
|e verfasserin
|4 aut
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|a Sorbo, Sergio
|e verfasserin
|4 aut
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|a Petraglia, Alessandro
|e verfasserin
|4 aut
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|a Degola, Francesca
|e verfasserin
|4 aut
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|a Bellini, Erika
|e verfasserin
|4 aut
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|a Bruno, Laura
|e verfasserin
|4 aut
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|a Varotto, Claudio
|e verfasserin
|4 aut
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|a Sanità di Toppi, Luigi
|e verfasserin
|4 aut
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| 773 |
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|i Enthalten in
|t Plant physiology and biochemistry : PPB
|d 1991
|g 127(2018) vom: 20. Juni, Seite 88-96
|w (DE-627)NLM098178261
|x 1873-2690
|7 nnns
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| 773 |
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|g volume:127
|g year:2018
|g day:20
|g month:06
|g pages:88-96
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|u http://dx.doi.org/10.1016/j.plaphy.2018.03.014
|3 Volltext
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|d 127
|j 2018
|b 20
|c 06
|h 88-96
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