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231225s2018 xx |||||o 00| ||eng c |
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|a 10.1016/j.plaphy.2018.02.023
|2 doi
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|a pubmed24n1224.xml
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|a (NLM)29525444
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|a (PII)S0981-9428(18)30075-5
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|a DE-627
|b ger
|c DE-627
|e rakwb
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|a eng
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|a Mattos, Bianca Braz
|e verfasserin
|4 aut
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|a A peptidogalactomannan isolated from Cladosporium herbarum induces defense-related genes in BY-2 tobacco cells
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|c 2018
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
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|2 rdamedia
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|a ƒa Online-Ressource
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|2 rdacarrier
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|a Date Completed 25.07.2018
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|a Date Revised 13.12.2023
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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|a Copyright © 2018 Elsevier Masson SAS. All rights reserved.
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|a Cladosporium herbarum is a plant pathogen associated with passion fruit scab and mild diseases in pea and soybean. In this study, a peptidogalactomannan (pGM) of C. herbarum mycelium was isolated and structurally characterized, and its role in plant-fungus interactions was evaluated. C. herbarum pGM is composed of carbohydrates (76%) and contains mannose, galactose and glucose as its main monosaccharides (molar ratio, 52:36:12). Methylation and 13C-nuclear magnetic resonance (13C-NMR) spectroscopy analysis have shown the presence of a main chain containing (1 → 6)-linked α-D-Manp residues, and β-D-Galf residues are present as (1 → 5)-interlinked side chains. β-Galactofuranose containing similar structures were characterized by our group in A. fumigatus, A. versicolor, A. flavus and C. resinae. Tobacco BY-2 cells were used as a model system to address the question of the role of C. herbarum pGM in cell viability and induction of the expression of plant defense-related genes. Native and partially acid hydrolyzed pGMs (lacking galactofuranosyl side-chain residues) were incubated with BY-2 cell suspensions at different concentrations. Cell viability drastically decreased after exposure to more than 400 μg ml-1 pGM; however no cell viability effect was observed after exposure to a partially acid hydrolyzed pGM. BY-2 cell contact with pGM strongly induce the expression of plant defense-related genes, such as phenylalanine ammonia lyase (PAL) and lipoxygenase (LOX), as well as the pathogen-related PR-1a, PR-2 and PR-3 genes, suggesting that pGM activates defense responses in tobacco cells. Interestingly, contact with partially hydrolyzed pGM also induced defense-related gene expression at earlier times than native pGM. These results show that the side chains of the (1 → 5)-linked β-D-galactofuranosyl units from pGM play an important role in the first line fungus-plant interactions mediating plant responses against C. herbarum. In addition, it was observed that pGM and/or C. herbarum conidia are able to induced HR when in contact with tobacco leaves and in vitro plantlets roots, producing necrotic lesions and peroxidase and NO burst, respectively
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|a Journal Article
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|a Cladosporium herbarum
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|a Glycoprotein
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|a Peptidogalactomannan
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|a Plant defense-related genes
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|a Montebianco, Caroline
|e verfasserin
|4 aut
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|a Romanel, Elisson
|e verfasserin
|4 aut
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|a da Franca Silva, Tatiane
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|a Bernabé, Renato Barroso
|e verfasserin
|4 aut
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|a Simas-Tosin, Fernanda
|e verfasserin
|4 aut
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|a Souza, Lauro M
|e verfasserin
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|a Sassaki, Guilherme L
|e verfasserin
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|a Vaslin, Maite F S
|e verfasserin
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|a Barreto-Bergter, Eliana
|e verfasserin
|4 aut
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|i Enthalten in
|t Plant physiology and biochemistry : PPB
|d 1991
|g 126(2018) vom: 01. Mai, Seite 206-216
|w (DE-627)NLM098178261
|x 1873-2690
|7 nnns
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|g volume:126
|g year:2018
|g day:01
|g month:05
|g pages:206-216
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|u http://dx.doi.org/10.1016/j.plaphy.2018.02.023
|3 Volltext
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