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231225s2018 xx |||||o 00| ||eng c |
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|a 10.1111/vcp.12598
|2 doi
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5 |
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|a pubmed25n0939.xml
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|a (DE-627)NLM281788189
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|a (NLM)29522252
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|a DE-627
|b ger
|c DE-627
|e rakwb
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| 041 |
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|a eng
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| 100 |
1 |
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|a Sawa, Mariko
|e verfasserin
|4 aut
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| 245 |
1 |
0 |
|a Rapid multiple immunofluorescent staining for the simultaneous detection of cytokeratin and vimentin in the cytology of canine tumors
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| 264 |
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1 |
|c 2018
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| 336 |
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|a Text
|b txt
|2 rdacontent
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| 337 |
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|a ƒaComputermedien
|b c
|2 rdamedia
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| 338 |
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|a ƒa Online-Ressource
|b cr
|2 rdacarrier
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| 500 |
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|a Date Completed 11.10.2018
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| 500 |
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|a Date Revised 11.10.2018
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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| 520 |
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|a © 2018 American Society for Veterinary Clinical Pathology.
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|a BACKGROUND: Immunocytochemistry (ICC) is utilized as an advanced technique in veterinary cytology. In tumor diagnosis, cytokeratin and vimentin are markers used to distinguish the origin of tumor cells. Standard enzyme-based ICC has limitations in clinical use; and therefore, more convenient and reliable methods are needed
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| 520 |
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|a OBJECTIVES: The purpose of this study was to develop a rapid multiple immunofluorescent (RMIF) detection method for dual cytokeratin and vimentin staining on cytology slides in dogs
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|a METHODS: Air-dried smear samples from solid tumors and sediments of pleural effusions were prepared from dogs (n = 14) that were admitted to the Veterinary Teaching Hospital, Kagoshima University, Japan. Mouse monoclonal anti-human cytokeratin (AE1/AE3) and rabbit monoclonal anti-human vimentin (SP20) antibodies were used as primary antibodies, followed by staining with Alexa Fluor-conjugated secondary antibodies. Staining using the RMIF method was compared with enzyme-based ICC staining
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| 520 |
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|a RESULTS: Rapid multiple immunofluorescent immunostaining was clear and specific in the evaluated smears, whereas the enzyme-based ICC showed nonspecific signals. By using the RMIF staining method, epithelial cells, mesenchymal cells, and mesothelial cells could be classified on a single smear of a pleural effusion. In smears of lymph nodes with epithelial tumor metastases, the RMIF method successfully detected metastatic epithelial tumor cells
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|a CONCLUSIONS: The RMIF method might be a useful tool for diagnostic cytology in veterinary medicine
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|a Comparative Study
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4 |
|a Journal Article
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| 650 |
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4 |
|a cytokeratin
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| 650 |
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4 |
|a cytology
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| 650 |
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4 |
|a dogs
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| 650 |
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4 |
|a immunofluorescence
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| 650 |
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4 |
|a vimentin
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| 650 |
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7 |
|a Biomarkers, Tumor
|2 NLM
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| 650 |
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7 |
|a Vimentin
|2 NLM
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| 650 |
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7 |
|a Keratins
|2 NLM
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| 650 |
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7 |
|a 68238-35-7
|2 NLM
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| 700 |
1 |
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|a Yabuki, Akira
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Kohyama, Moeko
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Miyoshi, Noriaki
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Yamato, Osamu
|e verfasserin
|4 aut
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| 773 |
0 |
8 |
|i Enthalten in
|t Veterinary clinical pathology
|d 1975
|g 47(2018), 2 vom: 20. Juni, Seite 326-332
|w (DE-627)NLM098159984
|x 1939-165X
|7 nnns
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| 773 |
1 |
8 |
|g volume:47
|g year:2018
|g number:2
|g day:20
|g month:06
|g pages:326-332
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| 856 |
4 |
0 |
|u http://dx.doi.org/10.1111/vcp.12598
|3 Volltext
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| 951 |
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|a AR
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| 952 |
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|d 47
|j 2018
|e 2
|b 20
|c 06
|h 326-332
|