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231225s2018 xx |||||o 00| ||eng c |
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|a 10.1002/adma.201702749
|2 doi
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|a pubmed24n0938.xml
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|a (NLM)29512198
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|a DE-627
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|c DE-627
|e rakwb
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|a eng
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| 100 |
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|a Molinaro, Roberto
|e verfasserin
|4 aut
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| 245 |
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|a Design and Development of Biomimetic Nanovesicles Using a Microfluidic Approach
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|c 2018
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
|b c
|2 rdamedia
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|a ƒa Online-Ressource
|b cr
|2 rdacarrier
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|a Date Completed 28.01.2019
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|a Date Revised 30.09.2020
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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|a © 2018 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
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|a The advancement of nanotechnology toward more sophisticated bioinspired approaches has highlighted the gap between the advantages of biomimetic and biohybrid platforms and the availability of manufacturing processes to scale up their production. Though the advantages of transferring biological features from cells to synthetic nanoparticles for drug delivery purposes have recently been reported, a standardizable, batch-to-batch consistent, scalable, and high-throughput assembly method is required to further develop these platforms. Microfluidics has offered a robust tool for the controlled synthesis of nanoparticles in a versatile and reproducible approach. In this study, the incorporation of membrane proteins within the bilayer of biomimetic nanovesicles (leukosomes) using a microfluidic-based platform is demonstrated. The physical, pharmaceutical, and biological properties of microfluidic-formulated leukosomes (called NA-Leuko) are characterized. NA-Leuko show extended shelf life and retention of the biological functions of donor cells (i.e., macrophage avoidance and targeting of inflamed vasculature). The NA approach represents a universal, versatile, robust, and scalable tool, which is extensively used for the assembly of lipid nanoparticles and adapted here for the manufacturing of biomimetic nanovesicles
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|a Journal Article
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|a bioinspired approach
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|a inflammation
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|a membrane protein incorporation
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4 |
|a microfluidics
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| 650 |
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4 |
|a molecular dynamics
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| 700 |
1 |
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|a Evangelopoulos, Michael
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Hoffman, Jessica R
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Corbo, Claudia
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Taraballi, Francesca
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Martinez, Jonathan O
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Hartman, Kelly A
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Cosco, Donato
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Costa, Giosue'
|e verfasserin
|4 aut
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| 700 |
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|a Romeo, Isabella
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Sherman, Michael
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Paolino, Donatella
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Alcaro, Stefano
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Tasciotti, Ennio
|e verfasserin
|4 aut
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| 773 |
0 |
8 |
|i Enthalten in
|t Advanced materials (Deerfield Beach, Fla.)
|d 1998
|g 30(2018), 15 vom: 07. Apr., Seite e1702749
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|g volume:30
|g year:2018
|g number:15
|g day:07
|g month:04
|g pages:e1702749
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|u http://dx.doi.org/10.1002/adma.201702749
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