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231225s2018 xx |||||o 00| ||eng c |
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|a 10.1016/j.plaphy.2018.01.023
|2 doi
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|a pubmed24n0938.xml
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|a (DE-627)NLM281522219
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|a (NLM)29494985
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|a (PII)S0981-9428(18)30029-9
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|a DE-627
|b ger
|c DE-627
|e rakwb
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|a eng
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| 100 |
1 |
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|a Mohammadi, Kourosh
|e verfasserin
|4 aut
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| 245 |
1 |
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|a Functional analysis of overexpressed PtDRS1 involved in abiotic stresses enhances growth in transgenic poplar
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|c 2018
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
|b c
|2 rdamedia
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|a ƒa Online-Ressource
|b cr
|2 rdacarrier
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|a Date Completed 25.07.2018
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|a Date Revised 30.09.2020
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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|a Copyright © 2018 Elsevier Masson SAS. All rights reserved.
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|a Drought and salinity are two main abiotic stressors that can disrupt plant growth and survival. Various biotechnological approaches have been used to alleviate the problem of drought stress by improving water stress resistance in forestry and agriculture. The drought sensitive 1 (DRS1) gene acts as a regulator of drought stress, identified in human, yeast and some model plants, such as Arabidopsis thaliana, but there have been no reports of DRS1 transformation in poplar plants to date. In this study, we transformed the DRS1 gene from Populus trichocarpa into Populus deltoides × Populus euramericana 'Nanlin895' using Agrobacterium tumefaciens-mediated transformation. We confirmed that the DRS1 gene was transformed into 'Nanlin895' poplar genomes using reverse transcription polymerase chain reaction (PCR), multiplex PCR, real-time PCR, and sodium dodecyl sulfate-polyacrylamide gel electrophoresis. All transformed and wild-type (WT) plants were then transferred into a greenhouse for complementary experiments. We analyzed the physiological and biochemical responses of transgenic plants under drought and salt stresses in the greenhouse, and the results were compared with control WT plants. Responses to abiotic stress were greater in transgenic plants compared with WT. Based on our results, introduction of the DRS1 gene into poplar 'Nanlin895' plants significantly enhanced the resistance of those plants to water deficit and high salinity, allowing higher growth rates of roots and shoots in those plants. Additionally, the clawed root rate increased in transformed poplars grown in culture media or in soil, and improved survival under drought and salt stress conditions
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|a Journal Article
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|a Abiotic stresses
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| 650 |
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4 |
|a DRS1
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|a Physiological and biochemical responses
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| 650 |
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|a Transformation
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| 650 |
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4 |
|a Transgenic poplar
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| 650 |
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7 |
|a Plant Proteins
|2 NLM
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| 700 |
1 |
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|a Movahedi, Ali
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Maleki, Samaneh Sadat
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Sun, Weibo
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Zhang, Jiaxin
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Almasi Zadeh Yaghuti, Amir
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Nourmohammadi, Saeed
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Zhuge, Qiang
|e verfasserin
|4 aut
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| 773 |
0 |
8 |
|i Enthalten in
|t Plant physiology and biochemistry : PPB
|d 1991
|g 126(2018) vom: 01. Mai, Seite 22-31
|w (DE-627)NLM098178261
|x 1873-2690
|7 nnns
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| 773 |
1 |
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|g volume:126
|g year:2018
|g day:01
|g month:05
|g pages:22-31
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|u http://dx.doi.org/10.1016/j.plaphy.2018.01.023
|3 Volltext
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|d 126
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|h 22-31
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