The effect of 1-methylcyclopropene (1-MCP) on expression of ethylene receptor genes in durian pulp during ripening

Copyright © 2018 Elsevier Masson SAS. All rights reserved.

Détails bibliographiques
Publié dans:Plant physiology and biochemistry : PPB. - 1991. - 125(2018) vom: 01. Apr., Seite 232-238
Auteur principal: Thongkum, Monthathip (Auteur)
Autres auteurs: Imsabai, Wachiraya, Burns, Parichart, McAtee, Peter A, Schaffer, Robert J, Allan, Andrew C, Ketsa, Saichol
Format: Article en ligne
Langue:English
Publié: 2018
Accès à la collection:Plant physiology and biochemistry : PPB
Sujets:Journal Article 1-MCP Durian Ethylene Ethylene receptor Ripening Signal transduction Cyclopropanes Plant Proteins Receptors, Cell Surface plus... ethylene receptors, plant 1-methylcyclopropane 594-11-6
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245 1 4 |a The effect of 1-methylcyclopropene (1-MCP) on expression of ethylene receptor genes in durian pulp during ripening 
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520 |a Rapid fruit ripening is a significant problem that limits the shelf life of durian, with ethylene having a major impact on the regulation of this event. Durian treated with ethephon ripened 3 d after treatment with increased pulp total soluble solids, ethylene production of the whole fruit and decreased pulp firmness compared to the control fruit. 1-MCP treatment delayed ripening by up to 9 d with inhibited accumulation of total soluble solids, color change, softening and ethylene production. Genes related to ethylene perception (DzETR1 and DzETR2) and the signaling pathway (DzCTR1, DzEIL1 and DzEIL2) in the pulp were investigated during this process, using qPCR to quantify changes in gene transcription. All candidate genes were significantly up-regulated in ripening durian pulp. Ethephon treatment increased the expression of DzETR1 and DzETR2 genes, while expression of DzCTR1, DzEIL1 and DzEIL2 were slightly affected. 1-MCP treatment significantly inhibited the expression of the DzETR2 and DzEIL1 genes. The promoters of DzETR2 genes were isolated and their activation by fruit transcription factors studied using transient expression in tobacco leaves. It was found that members of the kiwifruit and apple EIL1, EIL2 and EIL3 genes strongly activated the DzETR2 promoter. These results suggest that ethylene-induced ripening of durian is via the regulation of DzETR2 by EIL transcription factors 
650 4 |a Journal Article 
650 4 |a 1-MCP 
650 4 |a Durian 
650 4 |a Ethylene 
650 4 |a Ethylene receptor 
650 4 |a Ripening 
650 4 |a Signal transduction 
650 7 |a Cyclopropanes  |2 NLM 
650 7 |a Plant Proteins  |2 NLM 
650 7 |a Receptors, Cell Surface  |2 NLM 
650 7 |a ethylene receptors, plant  |2 NLM 
650 7 |a 1-methylcyclopropane  |2 NLM 
650 7 |a 594-11-6  |2 NLM 
700 1 |a Imsabai, Wachiraya  |e verfasserin  |4 aut 
700 1 |a Burns, Parichart  |e verfasserin  |4 aut 
700 1 |a McAtee, Peter A  |e verfasserin  |4 aut 
700 1 |a Schaffer, Robert J  |e verfasserin  |4 aut 
700 1 |a Allan, Andrew C  |e verfasserin  |4 aut 
700 1 |a Ketsa, Saichol  |e verfasserin  |4 aut 
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773 1 8 |g volume:125  |g year:2018  |g day:01  |g month:04  |g pages:232-238 
856 4 0 |u http://dx.doi.org/10.1016/j.plaphy.2018.02.004  |3 Volltext 
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