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231225s2018 xx |||||o 00| ||eng c |
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|a 10.1016/j.plaphy.2018.01.030
|2 doi
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|a pubmed24n0936.xml
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|a (DE-627)NLM280879385
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|a (NLM)29428820
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|a (PII)S0981-9428(18)30035-4
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|a DE-627
|b ger
|c DE-627
|e rakwb
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|a eng
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| 100 |
1 |
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|a Yu, Hai-Na
|e verfasserin
|4 aut
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|a Structural and biochemical characterization of the plant type III polyketide synthases of the liverwort Marchantia paleacea
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|c 2018
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
|b c
|2 rdamedia
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|a ƒa Online-Ressource
|b cr
|2 rdacarrier
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|a Date Completed 12.07.2018
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|a Date Revised 30.09.2020
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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|a Copyright © 2018 Elsevier Masson SAS. All rights reserved.
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|a Chalcone synthases (CHSs) of the type III polyketide synthases (PKSs), catalyze the formation of a tetraketide intermediate from a CoA-tethered starter and malonyl-CoA but use different cyclization mechanisms to produce distinct chemical scaffolds. Herein, we characterized CHS and CHS-like enzymes (designated MpCHS and MpCHSL1, 2 and 3) from Marchantia paleacea and determined the crystal structure of MpCHSL1. MpCHS catalyzed a Claisen condensation to form chalcone, while MpCHSLs catalyzed the formation of lactonized α-pyrones in vitro. Based on the structural, mutational and in vitro biochemical analyses, we established that MpCHSL1 is structurally and functionally closer to prototype CHS than stilbene synthase, and characterized the structural basis for the functional diversity of the type III PKSs. A chalcone-forming mutant of MpCHSL1 was build directed by the structural information. These findings pave the way for future studies to elucidate the functional diversity of type III PKSs in liverwort
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|a Journal Article
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|a Chalcone synthase
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|a Claisen condensation
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|a Crystal structure
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|a Lactonization
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|a Mutagenesis
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|a Type III polyketide synthase
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|a Plant Proteins
|2 NLM
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|a Polyketide Synthases
|2 NLM
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| 650 |
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|a 79956-01-7
|2 NLM
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| 700 |
1 |
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|a Liu, Xin-Yan
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Gao, Shuai
|e verfasserin
|4 aut
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| 700 |
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|a Sun, Bin
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Zheng, Hong-Bo
|e verfasserin
|4 aut
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| 700 |
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|a Ji, Mei
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Cheng, Ai-Xia
|e verfasserin
|4 aut
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| 700 |
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|a Lou, Hong-Xiang
|e verfasserin
|4 aut
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| 773 |
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|i Enthalten in
|t Plant physiology and biochemistry : PPB
|d 1991
|g 125(2018) vom: 01. Apr., Seite 95-105
|w (DE-627)NLM098178261
|x 1873-2690
|7 nnns
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| 773 |
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|g volume:125
|g year:2018
|g day:01
|g month:04
|g pages:95-105
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|u http://dx.doi.org/10.1016/j.plaphy.2018.01.030
|3 Volltext
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|d 125
|j 2018
|b 01
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|h 95-105
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