Overexpression of Fagopyrum tataricum FtbHLH2 enhances tolerance to cold stress in transgenic Arabidopsis

Copyright © 2018 Elsevier Masson SAS. All rights reserved.

Bibliographische Detailangaben
Veröffentlicht in:Plant physiology and biochemistry : PPB. - 1991. - 125(2018) vom: 01. Apr., Seite 85-94
1. Verfasser: Yao, Panfeng (VerfasserIn)
Weitere Verfasser: Sun, Zhaoxia, Li, Chenglei, Zhao, Xuerong, Li, Maofei, Deng, Renyu, Huang, Yunji, Zhao, Haixia, Chen, Hui, Wu, Qi
Format: Online-Aufsatz
Sprache:English
Veröffentlicht: 2018
Zugriff auf das übergeordnete Werk:Plant physiology and biochemistry : PPB
Schlagworte:Journal Article Chlorophyll fluorescence Cold stress Tartary buckwheat Transgenic A. thaliana bHLH protein Basic Helix-Loop-Helix Transcription Factors Plant Proteins
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500 |a Citation Status MEDLINE 
520 |a Copyright © 2018 Elsevier Masson SAS. All rights reserved. 
520 |a bHLH transcription factors play important roles in the abiotic stress response in plants, but their characteristics and functions in Tartary buckwheat (Fagopyrum tataricum), a traditional coarse cereal with a strong stress tolerance, haven't been sufficiently studied. Here, we found that the expression of a bHLH gene, FtbHLH2, was induced significantly by cold treatments in Tartary buckwheat seedlings. Subcellular localization indicated that FtbHLH2 localized in nucleus. Its overexpression in Arabidopsis increased tolerance to cold. The Arabidopsis plants overexpressing FtbHLH2 displayed higher root length and photosynthetic efficiency, and had lower malondialdehyde (MDA) and reactive oxygen species (ROS) after cold treatment compared to wild type (WT) plants. Meanwhile, the expression levels of some stress-related genes in transgenic plants were remarkably higher than that in wild type under normal and/or stress conditions. Furthermore, transgenic Arabidopsis lines with the FtbHLH2 promoter had higher GUS activity after cold stress. On the whole, the results suggest that FtbHLH2 may play a positive regulatory in cold stress of Tartary buckwheat 
650 4 |a Journal Article 
650 4 |a Chlorophyll fluorescence 
650 4 |a Cold stress 
650 4 |a Tartary buckwheat 
650 4 |a Transgenic A. thaliana 
650 4 |a bHLH protein 
650 7 |a Basic Helix-Loop-Helix Transcription Factors  |2 NLM 
650 7 |a Plant Proteins  |2 NLM 
700 1 |a Sun, Zhaoxia  |e verfasserin  |4 aut 
700 1 |a Li, Chenglei  |e verfasserin  |4 aut 
700 1 |a Zhao, Xuerong  |e verfasserin  |4 aut 
700 1 |a Li, Maofei  |e verfasserin  |4 aut 
700 1 |a Deng, Renyu  |e verfasserin  |4 aut 
700 1 |a Huang, Yunji  |e verfasserin  |4 aut 
700 1 |a Zhao, Haixia  |e verfasserin  |4 aut 
700 1 |a Chen, Hui  |e verfasserin  |4 aut 
700 1 |a Wu, Qi  |e verfasserin  |4 aut 
773 0 8 |i Enthalten in  |t Plant physiology and biochemistry : PPB  |d 1991  |g 125(2018) vom: 01. Apr., Seite 85-94  |w (DE-627)NLM098178261  |x 1873-2690  |7 nnns 
773 1 8 |g volume:125  |g year:2018  |g day:01  |g month:04  |g pages:85-94 
856 4 0 |u http://dx.doi.org/10.1016/j.plaphy.2018.01.028  |3 Volltext 
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