Standardized cloning vectors for protein production and generation of large gene libraries in Escherichia coli
Here, we modified the multiple cloning sites from commonly used expression vectors to create a new suite of cloning plasmids that simplify and speed up cloning procedures in Escherichia coli. Each of our standardized plasmids contains two BsaI restriction sites, allowing for highly efficient cloning...
| Veröffentlicht in: | BioTechniques. - 1991. - 64(2018), 1 vom: 01. Jan., Seite 24-26 |
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| Weitere Verfasser: | , , |
| Format: | Online-Aufsatz |
| Sprache: | English |
| Veröffentlicht: |
2018
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| Zugriff auf das übergeordnete Werk: | BioTechniques |
| Schlagworte: | Journal Article Research Support, Non-U.S. Gov't BsaI Escherichia coli Golden Gate cloning gene library protein production type II restriction enzymes DNA Restriction Enzymes EC 3.1.21.- |
| Online verfügbar |
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