General and facile purification of dye-labeled oligonucleotides by pH-controlled extraction
Previously, we reported a method for facile purification of oligonucleotides labeled with hydrophobic dyes, based on the solubility difference between the hydrophilic DNA and unreacted dye. Here, we present a new purification method applicable to any dye regardless of its hydrophobicity. We exploite...
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Bibliographische Detailangaben
| Veröffentlicht in: | BioTechniques. - 1991. - 64(2018), 1 vom: 01. Jan., Seite 21-23
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| 1. Verfasser: |
Bak, So Young
(VerfasserIn) |
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| Weitere Verfasser: |
Hwang, Jihee,
Bae, Sohyeon,
Lim, Soonkyu,
Kim, Younggyu,
Alzahrani, Khalid,
Wahab, Rizwan,
Alkhedhairy, Abdulaziz,
Kim, Seong Keun |
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| Format: | Online-Aufsatz
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| Sprache: | English |
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| Veröffentlicht: |
2018
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| Zugriff auf das übergeordnete Werk: | BioTechniques
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| Schlagworte: | Journal Article
Research Support, Non-U.S. Gov't
dye-labeled oligonucleotide purification
fluorescent dye pKa
free dyes extraction
Carbocyanines
Fluorescent Dyes
Oligonucleotides
Solutions
cyanine dye 5
mehr...
Water
059QF0KO0R
DNA
9007-49-2 |
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