An early-flowering einkorn wheat mutant with deletions of PHYTOCLOCK 1/LUX ARRHYTHMO and VERNALIZATION 2 exhibits a high level of VERNALIZATION 1 expression induced by vernalization

Copyright © 2018 Elsevier GmbH. All rights reserved.

Bibliographische Detailangaben
Veröffentlicht in:Journal of plant physiology. - 1979. - 222(2018) vom: 15. März, Seite 28-38
1. Verfasser: Nishiura, Aiko (VerfasserIn)
Weitere Verfasser: Kitagawa, Satoshi, Matsumura, Mina, Kazama, Yusuke, Abe, Tomoko, Mizuno, Nobuyuki, Nasuda, Shuhei, Murai, Koji
Format: Online-Aufsatz
Sprache:English
Veröffentlicht: 2018
Zugriff auf das übergeordnete Werk:Journal of plant physiology
Schlagworte:Journal Article Circadian clock gene Early-flowering Heavy-ion beam mutation Triticum monococcum Vernalization Wheat Plant Proteins
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245 1 3 |a An early-flowering einkorn wheat mutant with deletions of PHYTOCLOCK 1/LUX ARRHYTHMO and VERNALIZATION 2 exhibits a high level of VERNALIZATION 1 expression induced by vernalization 
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520 |a Copyright © 2018 Elsevier GmbH. All rights reserved. 
520 |a Using heavy-ion beam mutagenesis of Triticum monococcum strain KU104-1, we identified a mutant that shows extra early-flowering; it was named extra early-flowering 3 (exe3). Here, we carried out expression analyses of clock-related genes, clock downstream genes and photoperiod pathway genes, and found that the clock component gene PHYTOCLOCK 1/LUX ARRHYTHMO (PCL1/LUX) was not expressed in exe3 mutant plants. A PCR analysis of DNA markers indicated that the exe3 mutant had a deletion of wheat PCL1/LUX (WPCL1), and that the WPCL1 deletion was correlated with the mutant phenotype in the segregation line. We confirmed that the original strain KU104-1 carried a mutation that produced a null allele of a flowering repressor gene VERNALIZATION 2 (VRN2). As a result, the exe3 mutant has both WPCL1 and VRN2 loss-of-function mutations. Analysis of plant development in a growth chamber showed that vernalization treatment accelerated flowering time in the exe3 mutant under short day (SD) as well as long day (LD) conditions, and the early-flowering phenotype was correlated with the earlier up-regulation of VRN1. The deletion of WPCL1 affects the SD-specific expression patterns of some clock-related genes, clock downstream genes and photoperiod pathway genes, suggesting that the exe3 mutant causes a disordered SD response. The present study indicates that VRN1 expression is associated with the biological clock and the VRN1 up-regulation is not influenced by the presence or absence of VRN2 
650 4 |a Journal Article 
650 4 |a Circadian clock gene 
650 4 |a Early-flowering 
650 4 |a Heavy-ion beam mutation 
650 4 |a Triticum monococcum 
650 4 |a Vernalization 
650 4 |a Wheat 
650 7 |a Plant Proteins  |2 NLM 
700 1 |a Kitagawa, Satoshi  |e verfasserin  |4 aut 
700 1 |a Matsumura, Mina  |e verfasserin  |4 aut 
700 1 |a Kazama, Yusuke  |e verfasserin  |4 aut 
700 1 |a Abe, Tomoko  |e verfasserin  |4 aut 
700 1 |a Mizuno, Nobuyuki  |e verfasserin  |4 aut 
700 1 |a Nasuda, Shuhei  |e verfasserin  |4 aut 
700 1 |a Murai, Koji  |e verfasserin  |4 aut 
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773 1 8 |g volume:222  |g year:2018  |g day:15  |g month:03  |g pages:28-38 
856 4 0 |u http://dx.doi.org/10.1016/j.jplph.2018.01.002  |3 Volltext 
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