Suppression of a single BAHD gene in Setaria viridis causes large, stable decreases in cell wall feruloylation and increases biomass digestibility

Bibliographische Detailangaben
Veröffentlicht in:	The New phytologist. - 1979. - 218(2018), 1 vom: 15. Apr., Seite 81-93
1. Verfasser:	de Souza, Wagner R (VerfasserIn)
Weitere Verfasser:	Martins, Polyana K, Freeman, Jackie, Pellny, Till K, Michaelson, Louise V, Sampaio, Bruno L, Vinecky, Felipe, Ribeiro, Ana P, da Cunha, Barbara A D B, Kobayashi, Adilson K, de Oliveira, Patricia A, Campanha, Raquel B, Pacheco, Thályta F, Martarello, Danielly C I, Marchiosi, Rogério, Ferrarese-Filho, Osvaldo, Dos Santos, Wanderley D, Tramontina, Robson, Squina, Fabio M, Centeno, Danilo C, Gaspar, Marília, Braga, Marcia R, Tiné, Marco A S, Ralph, John, Mitchell, Rowan A C, Molinari, Hugo B C
Format:	Online-Aufsatz
Sprache:	English
Veröffentlicht:	2018
Zugriff auf das übergeordnete Werk:	The New phytologist
Schlagworte:	Journal Article Research Support, Non-U.S. Gov't cell wall acylation ferulic acid grass evolution hydroxycinnamates lignocellulosic feedstock Acids Coumaric Acids Xylans mehr... Lignin 9005-53-2 AVM951ZWST Coenzyme A-Transferases EC 2.8.3.-

Beschreibung
Zusammenfassung:	© 2017 The Authors. New Phytologist © 2017 New Phytologist Trust. Feruloylation of arabinoxylan (AX) in grass cell walls is a key determinant of recalcitrance to enzyme attack, making it a target for improvement of grass crops, and of interest in grass evolution. Definitive evidence on the genes responsible is lacking so we studied a candidate gene that we identified within the BAHD acyl-CoA transferase family. We used RNA interference (RNAi) silencing of orthologs in the model grasses Setaria viridis (SvBAHD01) and Brachypodium distachyon (BdBAHD01) and determined effects on AX feruloylation. Silencing of SvBAHD01 in Setaria resulted in a c. 60% decrease in AX feruloylation in stems consistently across four generations. Silencing of BdBAHD01 in Brachypodium stems decreased feruloylation much less, possibly due to higher expression of functionally redundant genes. Setaria SvBAHD01 RNAi plants showed: no decrease in total lignin, approximately doubled arabinose acylated by p-coumarate, changes in two-dimensional NMR spectra of unfractionated cell walls consistent with biochemical estimates, no effect on total biomass production and an increase in biomass saccharification efficiency of 40-60%. We provide the first strong evidence for a key role of the BAHD01 gene in AX feruloylation and demonstrate that it is a promising target for improvement of grass crops for biofuel, biorefining and animal nutrition applications
Beschreibung:	Date Completed 27.09.2019 Date Revised 05.11.2023 published: Print-Electronic CommentIn: New Phytol. 2018 Apr;218(1):5-7. - PMID 29488282 Citation Status MEDLINE
ISSN:	1469-8137
DOI:	10.1111/nph.14970