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231225s2018 xx |||||o 00| ||eng c |
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|a 10.1093/jxb/erx455
|2 doi
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|a pubmed25n0932.xml
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|a (NLM)29309624
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| 041 |
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|a eng
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| 100 |
1 |
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|a Bárány, Ivett
|e verfasserin
|4 aut
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| 245 |
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|a Autophagy is activated and involved in cell death with participation of cathepsins during stress-induced microspore embryogenesis in barley
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|c 2018
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
|b c
|2 rdamedia
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| 338 |
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|a ƒa Online-Ressource
|b cr
|2 rdacarrier
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| 500 |
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|a Date Completed 29.07.2019
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|a Date Revised 30.03.2022
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|a published: Print
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|a Citation Status MEDLINE
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|a Microspores are reprogrammed towards embryogenesis by stress. Many microspores die after this stress, limiting the efficiency of microspore embryogenesis. Autophagy is a degradation pathway that plays critical roles in stress response and cell death. In animals, cathepsins have an integral role in autophagy by degrading autophagic material; less is known in plants. Plant cathepsins are papain-like C1A cysteine proteases involved in many physiological processes, including programmed cell death. We have analysed the involvement of autophagy in cell death, in relation to cathepsin activation, during stress-induced microspore embryogenesis in Hordeum vulgare. After stress, reactive oxygen species (ROS) and cell death increased and autophagy was activated, including HvATG5 and HvATG6 up-regulation and increase of ATG5, ATG8, and autophagosomes. Concomitantly, cathepsin L/F-, B-, and H-like activities were induced, cathepsin-like genes HvPap-1 and HvPap-6 were up-regulated, and HvPap-1, HvPap-6, and HvPap-19 proteins increased and localized in the cytoplasm, resembling autophagy structures. Inhibitors of autophagy and cysteine proteases reduced cell death and promoted embryogenesis. The findings reveal a role for autophagy in stress-induced cell death during microspore embryogenesis, and the participation of cathepsins. Similar patterns of activation, expression, and localization suggest a possible connection between cathepsins and autophagy. The results open up new possibilities to enhance microspore embryogenesis efficiency with autophagy and/or cysteine protease modulators
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|a Journal Article
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|a Research Support, Non-U.S. Gov't
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|a Cathepsins
|2 NLM
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| 650 |
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|a EC 3.4.-
|2 NLM
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| 700 |
1 |
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|a Berenguer, Eduardo
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Solís, María-Teresa
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Pérez-Pérez, Yolanda
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Santamaría, M Estrella
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Crespo, José Luis
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Risueño, María C
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Díaz, Isabel
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Testillano, Pilar S
|e verfasserin
|4 aut
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| 773 |
0 |
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|i Enthalten in
|t Journal of experimental botany
|d 1985
|g 69(2018), 6 vom: 14. März, Seite 1387-1402
|w (DE-627)NLM098182706
|x 1460-2431
|7 nnns
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| 773 |
1 |
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|g volume:69
|g year:2018
|g number:6
|g day:14
|g month:03
|g pages:1387-1402
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| 856 |
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|u http://dx.doi.org/10.1093/jxb/erx455
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