Three UDP-xylose transporters participate in xylan biosynthesis by conveying cytosolic UDP-xylose into the Golgi lumen in Arabidopsis

UDP-xylose (UDP-Xyl) is synthesized by UDP-glucuronic acid decarboxylases, also termed UDP-Xyl synthases (UXSs). The Arabidopsis genome encodes six UXSs, which fall into two groups based upon their subcellular location: the Golgi lumen and the cytosol. The latter group appears to play an important r...

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Veröffentlicht in:Journal of experimental botany. - 1985. - 69(2018), 5 vom: 23. Feb., Seite 1125-1134
1. Verfasser: Zhao, Xianhai (VerfasserIn)
Weitere Verfasser: Liu, Nian, Shang, Na, Zeng, Wei, Ebert, Berit, Rautengarten, Carsten, Zeng, Qing-Yin, Li, Huiling, Chen, Xiaoyang, Beahan, Cherie, Bacic, Antony, Heazlewood, Joshua L, Wu, Ai-Min
Format: Online-Aufsatz
Sprache:English
Veröffentlicht: 2018
Zugriff auf das übergeordnete Werk:Journal of experimental botany
Schlagworte:Journal Article Research Support, Non-U.S. Gov't AT1G06890 protein, Arabidopsis AT2G28315 protein, Arabidopsis AT2G30460 protein, Arabidopsis Arabidopsis Proteins Nucleoside Transport Proteins Xylans Uridine Diphosphate Xylose 3616-06-6 mehr... Xylose A1TA934AKO
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100 1 |a Zhao, Xianhai  |e verfasserin  |4 aut 
245 1 0 |a Three UDP-xylose transporters participate in xylan biosynthesis by conveying cytosolic UDP-xylose into the Golgi lumen in Arabidopsis 
264 1 |c 2018 
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500 |a Date Revised 11.12.2024 
500 |a published: Print 
500 |a CommentIn: J Exp Bot. 2018 Feb 23;69(5):905-907. doi: 10.1093/jxb/ery026. - PMID 29796610 
500 |a Citation Status MEDLINE 
520 |a UDP-xylose (UDP-Xyl) is synthesized by UDP-glucuronic acid decarboxylases, also termed UDP-Xyl synthases (UXSs). The Arabidopsis genome encodes six UXSs, which fall into two groups based upon their subcellular location: the Golgi lumen and the cytosol. The latter group appears to play an important role in xylan biosynthesis. Cytosolic UDP-Xyl is transported into the Golgi lumen by three UDP-Xyl transporters (UXT1, 2, and 3). However, while single mutants affected in the UDP-Xyl transporter 1 (UXT1) showed a substantial reduction in cell wall xylose content, a double mutant affected in UXT2 and UXT3 had no obvious effect on cell wall xylose deposition. This prompted us to further investigate redundancy among the members of the UXT family. Multiple uxt mutants were generated, including a triple mutant, which exhibited collapsed vessels and reduced cell wall thickness in interfascicular fiber cells. Monosaccharide composition, molecular weight, nuclear magnetic resonance, and immunolabeling studies demonstrated that both xylan biosynthesis (content) and fine structure were significantly affected in the uxt triple mutant, leading to phenotypes resembling those of the irx mutants. Pollination was also impaired in the uxt triple mutant, likely due to reduced filament growth and anther dehiscence caused by alterations in the composition of the cell walls. Moreover, analysis of the nucleotide sugar composition of the uxt mutants indicated that nucleotide sugar interconversion is influenced by the cytosolic UDP-Xyl pool within the cell. Taken together, our results underpin the physiological roles of the UXT family in xylan biosynthesis and provide novel insights into the nucleotide sugar metabolism and trafficking in plants 
650 4 |a Journal Article 
650 4 |a Research Support, Non-U.S. Gov't 
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700 1 |a Liu, Nian  |e verfasserin  |4 aut 
700 1 |a Shang, Na  |e verfasserin  |4 aut 
700 1 |a Zeng, Wei  |e verfasserin  |4 aut 
700 1 |a Ebert, Berit  |e verfasserin  |4 aut 
700 1 |a Rautengarten, Carsten  |e verfasserin  |4 aut 
700 1 |a Zeng, Qing-Yin  |e verfasserin  |4 aut 
700 1 |a Li, Huiling  |e verfasserin  |4 aut 
700 1 |a Chen, Xiaoyang  |e verfasserin  |4 aut 
700 1 |a Beahan, Cherie  |e verfasserin  |4 aut 
700 1 |a Bacic, Antony  |e verfasserin  |4 aut 
700 1 |a Heazlewood, Joshua L  |e verfasserin  |4 aut 
700 1 |a Wu, Ai-Min  |e verfasserin  |4 aut 
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