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231225s2018 xx |||||o 00| ||eng c |
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|a 10.1111/nph.14971
|2 doi
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|a pubmed25n0931.xml
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|a (DE-627)NLM279607806
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|a (NLM)29297591
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|a DE-627
|b ger
|c DE-627
|e rakwb
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|a eng
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|a Op De Beeck, Michiel
|e verfasserin
|4 aut
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|a Fenton reaction facilitates organic nitrogen acquisition by an ectomycorrhizal fungus
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|c 2018
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
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|2 rdamedia
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|a ƒa Online-Ressource
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|2 rdacarrier
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|a Date Completed 27.09.2019
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|a Date Revised 30.09.2020
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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|a © 2018 The Authors. New Phytologist © 2018 New Phytologist Trust.
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|a Boreal trees rely on their ectomycorrhizal fungal symbionts to acquire growth-limiting nutrients, such as nitrogen (N), which mainly occurs as proteins complexed in soil organic matter (SOM). The mechanisms for liberating this N are unclear as ectomycorrhizal fungi have lost many genes encoding lignocellulose-degrading enzymes present in their saprotrophic ancestors. We hypothesized that hydroxyl radicals (˙ OH), produced by the ectomycorrhizal fungus Paxillus involutus during growth on SOM, are involved in liberating organic N. Paxillus involutus was grown for 7 d on N-containing or N-free substrates that represent major organic compounds of SOM. ˙ OH production, ammonium assimilation, and proteolytic activity were measured daily. ˙ OH production was strongly induced when P. involutus switched from ammonium to protein as the main N source. Extracellular proteolytic activity was initiated shortly after the oxidation. Oxidized protein substrates induced higher proteolytic activity than unmodified proteins. Dynamic modeling predicted that ˙ OH production occurs in a burst, regulated mainly by ammonium and ferric iron concentrations. We propose that the production of ˙ OH and extracellular proteolytic enzymes are regulated by similar nutritional signals. Oxidation works in concert with proteolysis, improving N liberation from proteins in SOM. Organic N mining by ectomycorrhizal fungi has, until now, only been attributed to proteolysis
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|a Journal Article
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|a Research Support, Non-U.S. Gov't
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|a Paxillus involutus
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|a Fenton reaction
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|a nitrogen (N)
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|a proteolysis
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|a soil organic matter (SOM)
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|a Fenton's reagent
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|a Fungal Proteins
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|a Organic Chemicals
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|a Aspartic Acid
|2 NLM
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|a 30KYC7MIAI
|2 NLM
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|a Hydroxyl Radical
|2 NLM
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|a 3352-57-6
|2 NLM
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|a Hydrogen Peroxide
|2 NLM
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|a BBX060AN9V
|2 NLM
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|a Iron
|2 NLM
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|a E1UOL152H7
|2 NLM
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|a Nitrogen
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|a N762921K75
|2 NLM
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|a Troein, Carl
|e verfasserin
|4 aut
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|a Peterson, Carsten
|e verfasserin
|4 aut
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|a Persson, Per
|e verfasserin
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|a Tunlid, Anders
|e verfasserin
|4 aut
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| 773 |
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|i Enthalten in
|t The New phytologist
|d 1989
|g 218(2018), 1 vom: 15. Apr., Seite 335-343
|w (DE-627)NLM09818248X
|x 1469-8137
|7 nnns
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| 773 |
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|g volume:218
|g year:2018
|g number:1
|g day:15
|g month:04
|g pages:335-343
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|u http://dx.doi.org/10.1111/nph.14971
|3 Volltext
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|d 218
|j 2018
|e 1
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|c 04
|h 335-343
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