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231225s2018 xx |||||o 00| ||eng c |
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|a 10.1093/jxb/erx426
|2 doi
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|a pubmed25n0931.xml
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|a DE-627
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|e rakwb
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|a eng
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|a Gelová, Zuzana
|e verfasserin
|4 aut
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|a Antibody-mediated modulation of cytokinins in tobacco
|b organ-specific changes in cytokinin homeostasis
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|c 2018
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
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|2 rdamedia
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|a ƒa Online-Ressource
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|2 rdacarrier
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|a Date Completed 14.05.2019
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|a Date Revised 13.12.2023
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|a published: Print
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|a Citation Status MEDLINE
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|a © The Author(s) 2017. Published by Oxford University Press on behalf of the Society for Experimental Biology. All rights reserved. For permissions, please email: journals.permissionsoup.com.
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|a Cytokinins comprise a group of phytohormones with an organ-specific mode of action. Although the mechanisms controlling the complex networks of cytokinin metabolism are partially known, the role of individual cytokinin types in the maintenance of cytokinin homeostasis remains unclear. Utilizing the overproduction of single-chain Fv antibodies selected for their ability to bind trans-zeatin riboside and targeted to the endoplasmic reticulum, we post-synthetically modulated cytokinin ribosides, the proposed transport forms of cytokinins. We observed asymmetric activity of cytokinin biosynthetic genes and cytokinin distribution in wild-type tobacco seedlings with higher cytokinin abundance in the root than in the shoot. Antibody-mediated modulation of cytokinin ribosides further enhanced the relative cytokinin abundance in the roots and induced cytokinin-related phenotypes in an organ-specific manner. The activity of cytokinin oxidase/dehydrogenase in the roots was strongly up-regulated in response to antibody-mediated formation of the cytokinin pool in the endoplasmic reticulum. However, we only detected a slight decrease in the root cytokinin levels. In contrast, a significant decrease of cytokinins occurred in the shoot. We suggest the roots as the main site of cytokinin biosynthesis in tobacco seedlings. Conversely, cytokinin levels in the shoot seem to depend largely on long-range transport of cytokinin ribosides from the root and their subsequent metabolic activation
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|a Journal Article
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|a Research Support, Non-U.S. Gov't
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|a Antibody-mediated modulation
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|a CKX
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|a biosynthesis
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|a cytokinin
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|a homeostasis
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|a organ specificity
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|a tobacco
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|a Cytokinins
|2 NLM
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|a Plant Growth Regulators
|2 NLM
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|a Plantibodies
|2 NLM
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|a Isopentenyladenosine
|2 NLM
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|a 7724-76-7
|2 NLM
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|a zeatin riboside
|2 NLM
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|a 7LG4D082A9
|2 NLM
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|a Ten Hoopen, Petra
|e verfasserin
|4 aut
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|a Novák, Ondrej
|e verfasserin
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|a Motyka, Václav
|e verfasserin
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|a Pernisová, Markéta
|e verfasserin
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|a Dabravolski, Siarhei
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|a Didi, Vojtech
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|a Tillack, Isolde
|e verfasserin
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|a Okleštková, Jana
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|a Strnad, Miroslav
|e verfasserin
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|a Hause, Bettina
|e verfasserin
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|a Haruštiaková, Danka
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|a Conrad, Udo
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|a Janda, Lubomír
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|a Hejátko, Jan
|e verfasserin
|4 aut
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| 773 |
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|i Enthalten in
|t Journal of experimental botany
|d 1985
|g 69(2018), 3 vom: 23. Jan., Seite 441-454
|w (DE-627)NLM098182706
|x 1460-2431
|7 nnas
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| 773 |
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|g volume:69
|g year:2018
|g number:3
|g day:23
|g month:01
|g pages:441-454
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|u http://dx.doi.org/10.1093/jxb/erx426
|3 Volltext
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