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231225s2018 xx |||||o 00| ||eng c |
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|a 10.1111/nph.14964
|2 doi
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|a pubmed24n1223.xml
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|a DE-627
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|e rakwb
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|a eng
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1 |
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|a Zou, Shenghao
|e verfasserin
|4 aut
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| 245 |
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|a The NB-LRR gene Pm60 confers powdery mildew resistance in wheat
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|c 2018
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| 336 |
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
|b c
|2 rdamedia
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| 338 |
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|a ƒa Online-Ressource
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|2 rdacarrier
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|a Date Completed 27.09.2019
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|a Date Revised 13.12.2023
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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|a © 2017 The Authors. New Phytologist © 2017 New Phytologist Trust.
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|a Powdery mildew is one of the most devastating diseases of wheat. To date, few powdery mildew resistance genes have been cloned from wheat due to the size and complexity of the wheat genome. Triticum urartu is the progenitor of the A genome of wheat and is an important source for powdery mildew resistance genes. Using molecular markers designed from scaffolds of the sequenced T. urartu accession and standard map-based cloning, a powdery mildew resistance locus was mapped to a 356-kb region, which contains two nucleotide-binding and leucine-rich repeat domain (NB-LRR) protein-encoding genes. Virus-induced gene silencing, single-cell transient expression, and stable transformation assays demonstrated that one of these two genes, designated Pm60, confers resistance to powdery mildew. Overexpression of full-length Pm60 and two allelic variants in Nicotiana benthamiana leaves induced hypersensitive cell death response, but expression of the coiled-coil domain alone was insufficient to induce hypersensitive response. Yeast two-hybrid, bimolecular fluorescence complementation and luciferase complementation imaging assays showed that Pm60 protein interacts with its neighboring NB-containing protein, suggesting that they might be functionally related. The identification and cloning of this novel wheat powdery mildew resistance gene will facilitate breeding for disease resistance in wheat
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|a Journal Article
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|a Research Support, Non-U.S. Gov't
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|a Triticum aestivum
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| 650 |
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|a Triticum urartu
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| 650 |
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4 |
|a Blumeria graminis f. sp. tritici (Bgt)
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|a NB-LRR
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| 650 |
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4 |
|a Pm60
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| 650 |
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|a powdery mildew
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| 650 |
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|a wheat
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| 650 |
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|a Plant Proteins
|2 NLM
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| 700 |
1 |
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|a Wang, Huan
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Li, Yiwen
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Kong, Zhaosheng
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Tang, Dingzhong
|e verfasserin
|4 aut
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| 773 |
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|i Enthalten in
|t The New phytologist
|d 1979
|g 218(2018), 1 vom: 05. Apr., Seite 298-309
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|x 1469-8137
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|g volume:218
|g year:2018
|g number:1
|g day:05
|g month:04
|g pages:298-309
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|u http://dx.doi.org/10.1111/nph.14964
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