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231225s2018 xx |||||o 00| ||eng c |
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|a 10.1016/j.jplph.2017.12.014
|2 doi
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|a pubmed24n1223.xml
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|a (DE-627)NLM279406215
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|a (NLM)29277027
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|a (PII)S0176-1617(17)30310-3
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|a DE-627
|b ger
|c DE-627
|e rakwb
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|a eng
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| 100 |
1 |
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|a Li, Gui
|e verfasserin
|4 aut
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|a Non-plastidial expression of a synthetic insect geranyl pyrophosphate synthase effectively increases tobacco plant biomass
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|c 2018
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
|b c
|2 rdamedia
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|a ƒa Online-Ressource
|b cr
|2 rdacarrier
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|a Date Completed 31.07.2018
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|a Date Revised 13.12.2023
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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|a Copyright © 2017 Elsevier GmbH. All rights reserved.
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|a Designing effective synthetic genes of interest is a fundamental step in plant synthetic biology for biomass. Geranyl pyrophosphate (diphosphate) synthase (GPPS) catalyzes a bottleneck step toward terpenoid metabolism. We previously designed and synthesized a plant (Arabidopsis thaliana)-insect (Myzus persicae, Mp) GPPS- human influenza hemagglutinin (HA) cDNA, namely PTP-MpGPPS-HA (or PTP-sMpGPPS-HA, s: synthetic), to localize the protein in plastids and improve plant biomass. To better understand the effects of different subcellular localizations on plant performance, herein we report PTP-sMpGPPS-HA re-design to synthesize a new MpGPPS-HA cDNA, namely sMpGPPS-HA, to express a non-plastidial sMpGPPS-HA protein. The sMpGPPS-HA cDNA driven by a 2 × S 35S promoter was introduced into Nicotiana tabacum Xanthi. PTP-MpGPPS-HA and PMDC84 vector transgenic plants were also generated as positive and negative controls, respectively. Eighteen to twenty transgenic T0 lines were generated for each sMpGPPS-HA, PTP-sMpGPPS-HA, and PMDC84. Transcriptional genotyping analysis demonstrated the expression of sMpGPPS-HA in transgenic plants. Confocal microscopy analysis of transgenic progeny demonstrated the non-plastidial localization of sMpGPPS-HA. Growth of T1 transgenic and wild-type control plants showed that the expression of sMpGPPS-HA effectively increased plant height by 50-80%, leaf numbers and sizes, and dry biomass by 60-80%. Calculation of the vegetative growth rates showed that the expression of sMpGPPS-HA increased plant height each week. Moreover, sMpGPPS-HA expression promoted early flowering and reduced leaf carotenoid levels. In conclusion, non-plastidial expression of the novel sMpGPPS-HA was effective for improving tobacco growth and biomass. Our data indicate that research examining different subcellular localizations facilitates a better understanding of in planta functions of proteins encoded by synthetic cDNAs
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|a Journal Article
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|a Metabolic engineering
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|a Plant biomass
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|a Plant synthetic biology
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|a Synthetic geranyl pyrophosphate synthase
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| 650 |
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|a Insect Proteins
|2 NLM
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| 700 |
1 |
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|a Xi, Jing
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Ji, Xiaoming
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Li, Ming-Zhuo
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Xie, De-Yu
|e verfasserin
|4 aut
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| 773 |
0 |
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|i Enthalten in
|t Journal of plant physiology
|d 1979
|g 221(2018) vom: 09. Feb., Seite 144-155
|w (DE-627)NLM098174622
|x 1618-1328
|7 nnns
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| 773 |
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|g volume:221
|g year:2018
|g day:09
|g month:02
|g pages:144-155
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|u http://dx.doi.org/10.1016/j.jplph.2017.12.014
|3 Volltext
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