Morphological defects of sperm and their association with motility, fertility, and hatchability in four Korean native chicken breeds

OBJECTIVE: This study was conducted to compare morphological defects, viability, motility (MOT), fertility (F), and hatchability (H) in four Korean native chicken breeds (KNCBs), and to evaluate whether defective segments of spermatozoa are associated with MOT, F, and H

Bibliographische Detailangaben
Veröffentlicht in:Asian-Australasian journal of animal sciences. - 1998. - 31(2018), 8 vom: 15. Aug., Seite 1160-1168
1. Verfasser: Feyisa, Shiferaw Garoma (VerfasserIn)
Weitere Verfasser: Park, Young Hyun, Kim, Young Min, Lee, Bo Ram, Jung, Kyung Min, Choi, Seong Bok, Cho, Chang Yeon, Han, Jae Yong
Format: Online-Aufsatz
Sprache:English
Veröffentlicht: 2018
Zugriff auf das übergeordnete Werk:Asian-Australasian journal of animal sciences
Schlagworte:Journal Article Hatchability Morphological Defect Motility Native Chicken Fertility
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245 1 0 |a Morphological defects of sperm and their association with motility, fertility, and hatchability in four Korean native chicken breeds 
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520 |a OBJECTIVE: This study was conducted to compare morphological defects, viability, motility (MOT), fertility (F), and hatchability (H) in four Korean native chicken breeds (KNCBs), and to evaluate whether defective segments of spermatozoa are associated with MOT, F, and H 
520 |a METHODS: Four KNCBs, including Korean Ogye (KO), Hwangbong (HB), Hyunin Black (HH), and Hoengseong Yakdak (HY) were used. White Leghorn (WL) was used as a control. Nine cocks from each breed were randomly assigned into three groups. Semen was collected by abdominal massage method. Eosin-nigrosin staining method was used to identify live-dead spermatozoa. Different segments and specific morphological defects of spermatozoa were identified using 4', 6-diamidino-2-phenylidole and MitoTracker Red CMXRos. F and H rates were evaluated following artificial insemination (AI) 
520 |a RESULTS: KO had the highest MOT rate compared to HY. Viable normal sperm rates of KO and HH were high and comparable with WL. HY spermatozoa had the highest viable abnormal sperm (VAS) or morphological defect rate followed by HB. Likewise, HB spermatozoa had the highest dead sperm (dead) rate compared to KO, HY, and WL. Bent, coiled, detached, broken, and knotted were common identified specific morphological defects for all breeds. Most morphological defects were at the head and tail in all breeds. VAS showed strong negative correlation with MOT (r = -0.697) and F (r = -0.609). Similarly, defective tail was negatively correlated with MOT (r = -0.587), F (r = -0.797), and H (r = -0.448). The F and H rates of KO and WL were comparable 
520 |a CONCLUSION: These data indicate that most identified specific morphological defects are at the head and tail. VAS and defective tail were associated with poor motility, F, and H. KNCBs showed more morphological defects than WL. Finally, these results will facilitate successful AI and semen cryopreservation 
650 4 |a Journal Article 
650 4 |a Hatchability 
650 4 |a Morphological Defect 
650 4 |a Motility 
650 4 |a Native Chicken 
650 4 |a Fertility 
700 1 |a Park, Young Hyun  |e verfasserin  |4 aut 
700 1 |a Kim, Young Min  |e verfasserin  |4 aut 
700 1 |a Lee, Bo Ram  |e verfasserin  |4 aut 
700 1 |a Jung, Kyung Min  |e verfasserin  |4 aut 
700 1 |a Choi, Seong Bok  |e verfasserin  |4 aut 
700 1 |a Cho, Chang Yeon  |e verfasserin  |4 aut 
700 1 |a Han, Jae Yong  |e verfasserin  |4 aut 
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