Star-Shaped Thermoresponsive Polymers with Various Functional Groups for Cell Sheet Engineering

This study demonstrates the facile preparation of poly(N-isopropylacrylamide) (PNIPAM)-immobilized Petri dishes by drop-casting a star-shaped copolymer of hyperbranched polystyrene (HBPS) possessing PNIPAM arms (HBPS-g-PNIPAM) functionalized with polar groups. HBPS was synthesized via reversible add...

Ausführliche Beschreibung

Bibliographische Detailangaben
Veröffentlicht in:Langmuir : the ACS journal of surfaces and colloids. - 1992. - 34(2018), 2 vom: 16. Jan., Seite 653-662
1. Verfasser: Sudo, Yu (VerfasserIn)
Weitere Verfasser: Kawai, Ryuki, Sakai, Hideaki, Kikuchi, Ryohei, Nabae, Yuta, Hayakawa, Teruaki, Kakimoto, Masa-Aki
Format: Online-Aufsatz
Sprache:English
Veröffentlicht: 2018
Zugriff auf das übergeordnete Werk:Langmuir : the ACS journal of surfaces and colloids
Schlagworte:Journal Article
LEADER 01000naa a22002652 4500
001 NLM279221843
003 DE-627
005 20231225022451.0
007 cr uuu---uuuuu
008 231225s2018 xx |||||o 00| ||eng c
024 7 |a 10.1021/acs.langmuir.7b04213  |2 doi 
028 5 2 |a pubmed24n0930.xml 
035 |a (DE-627)NLM279221843 
035 |a (NLM)29257892 
040 |a DE-627  |b ger  |c DE-627  |e rakwb 
041 |a eng 
100 1 |a Sudo, Yu  |e verfasserin  |4 aut 
245 1 0 |a Star-Shaped Thermoresponsive Polymers with Various Functional Groups for Cell Sheet Engineering 
264 1 |c 2018 
336 |a Text  |b txt  |2 rdacontent 
337 |a ƒaComputermedien  |b c  |2 rdamedia 
338 |a ƒa Online-Ressource  |b cr  |2 rdacarrier 
500 |a Date Completed 13.04.2018 
500 |a Date Revised 13.04.2018 
500 |a published: Print-Electronic 
500 |a Citation Status PubMed-not-MEDLINE 
520 |a This study demonstrates the facile preparation of poly(N-isopropylacrylamide) (PNIPAM)-immobilized Petri dishes by drop-casting a star-shaped copolymer of hyperbranched polystyrene (HBPS) possessing PNIPAM arms (HBPS-g-PNIPAM) functionalized with polar groups. HBPS was synthesized via reversible addition-fragmentation chain transfer (RAFT) self-condensing vinyl polymerization (SCVP), and HBPS polymers with different terminal structures were prepared by changing the monomer structure. HBPS-g-PNIPAM was synthesized by the grafting of PNIPAM from each terminal of HBPS. To tune the cell adhesion and detachment properties, polar functional groups such as carboxylic acid and dimethylamino groups were introduced to HBPS-g-PNIPAM. Based on surface characterization using scanning transmission electron microscopy (STEM), X-ray photoelectron spectroscopy (XPS), and contact angle measurements, the advantage of the hyperbranched structure for the PNIPAM immobilization was evident in terms of the uniformity, stability, and thermoresponsiveness. Successful cell sheet harvesting was demonstrated on dishes coated with HBPS-g-PNIPAM. In addition, the cell adhesion and detachment properties could be tuned by the introduction of polar functional groups 
650 4 |a Journal Article 
700 1 |a Kawai, Ryuki  |e verfasserin  |4 aut 
700 1 |a Sakai, Hideaki  |e verfasserin  |4 aut 
700 1 |a Kikuchi, Ryohei  |e verfasserin  |4 aut 
700 1 |a Nabae, Yuta  |e verfasserin  |4 aut 
700 1 |a Hayakawa, Teruaki  |e verfasserin  |4 aut 
700 1 |a Kakimoto, Masa-Aki  |e verfasserin  |4 aut 
773 0 8 |i Enthalten in  |t Langmuir : the ACS journal of surfaces and colloids  |d 1992  |g 34(2018), 2 vom: 16. Jan., Seite 653-662  |w (DE-627)NLM098181009  |x 1520-5827  |7 nnns 
773 1 8 |g volume:34  |g year:2018  |g number:2  |g day:16  |g month:01  |g pages:653-662 
856 4 0 |u http://dx.doi.org/10.1021/acs.langmuir.7b04213  |3 Volltext 
912 |a GBV_USEFLAG_A 
912 |a SYSFLAG_A 
912 |a GBV_NLM 
912 |a GBV_ILN_22 
912 |a GBV_ILN_350 
912 |a GBV_ILN_721 
951 |a AR 
952 |d 34  |j 2018  |e 2  |b 16  |c 01  |h 653-662