A dominant-interfering camta3 mutation compromises primary transcriptional outputs mediated by both cell surface and intracellular immune receptors in Arabidopsis thaliana

Bibliographische Detailangaben
Veröffentlicht in:	The New phytologist. - 1979. - 217(2018), 4 vom: 11. März, Seite 1667-1680
1. Verfasser:	Jacob, Florence (VerfasserIn)
Weitere Verfasser:	Kracher, Barbara, Mine, Akira, Seyfferth, Carolin, Blanvillain-Baufumé, Servane, Parker, Jane E, Tsuda, Kenichi, Schulze-Lefert, Paul, Maekawa, Takaki
Format:	Online-Aufsatz
Sprache:	English
Veröffentlicht:	2018
Zugriff auf das übergeordnete Werk:	The New phytologist
Schlagworte:	Journal Article Research Support, Non-U.S. Gov't Arabidopsis thaliana calmodulin-binding transcription activator (CAMTA) cross-tolerance effector-triggered immunity (ETI) immediate early genes nucleotide-binding domain and LRR-containing proteins (NLRs) pattern-triggered immunity (PTI) transcriptional responses mehr... AT2G22300 protein, Arabidopsis Arabidopsis Proteins NLR Proteins Receptors, Immunologic Receptors, Pattern Recognition Transcription Factors

Beschreibung
Zusammenfassung:	© 2017 The Authors. New Phytologist © 2017 New Phytologist Trust. Pattern recognition receptors (PRRs) and nucleotide-binding domain and leucine-rich repeat (LRR)-containing proteins (NLRs) initiate pattern-triggered immunity (PTI) and effector-triggered immunity (ETI), respectively, each associated with the activation of an overlapping set of defence genes. The regulatory mechanism behind this convergence of PTI- and ETI-mediated defence gene induction remains elusive. We generated transgenic Arabidopsis plants that enable conditional NLR activation without pathogen infection to dissect NLR- and PRR-mediated transcriptional signals. A comparative analysis of over 40 transcriptome datasets linked calmodulin-binding transcription activators (CAMTAs) to the activation of overlapping defence genes in PTI and ETI. We used a dominant camta3 mutant (camta3-D) to assess CAMTA functions in the corresponding transcriptional regulation. Transcriptional regulation by NLRs, although highly similar to PTI responses, can be established independently of pathogen-associated molecular pattern (PAMP) perception, defence phytohormones and host cell death. Conditional expression of the N-terminal coiled-coil domain of the barley MLA (Mildew resistance locus A) NLR is sufficient to trigger similar transcriptional reprogramming as full-length NLRs. CAMTA-binding motifs are overrepresented in the 5' regulatory regions of the identified primary immune response genes, consistent with their altered expression and disease resistance responses in camta3-D plants. We propose that CAMTA-mediated transcriptional regulation defines an early convergence point in NLR- and PRR-mediated signalling
Beschreibung:	Date Completed 13.09.2019 Date Revised 18.04.2022 published: Print-Electronic Citation Status MEDLINE
ISSN:	1469-8137
DOI:	10.1111/nph.14943