|
|
|
|
| LEADER |
01000naa a22002652 4500 |
| 001 |
NLM278262422 |
| 003 |
DE-627 |
| 005 |
20231225020142.0 |
| 007 |
cr uuu---uuuuu |
| 008 |
231225s2017 xx |||||o 00| ||eng c |
| 024 |
7 |
|
|a 10.1021/acs.langmuir.7b03742
|2 doi
|
| 028 |
5 |
2 |
|a pubmed24n0927.xml
|
| 035 |
|
|
|a (DE-627)NLM278262422
|
| 035 |
|
|
|a (NLM)29160078
|
| 040 |
|
|
|a DE-627
|b ger
|c DE-627
|e rakwb
|
| 041 |
|
|
|a eng
|
| 100 |
1 |
|
|a Oluwole, Abraham Olusegun
|e verfasserin
|4 aut
|
| 245 |
1 |
0 |
|a Formation of Lipid-Bilayer Nanodiscs by Diisobutylene/Maleic Acid (DIBMA) Copolymer
|
| 264 |
|
1 |
|c 2017
|
| 336 |
|
|
|a Text
|b txt
|2 rdacontent
|
| 337 |
|
|
|a ƒaComputermedien
|b c
|2 rdamedia
|
| 338 |
|
|
|a ƒa Online-Ressource
|b cr
|2 rdacarrier
|
| 500 |
|
|
|a Date Completed 18.01.2019
|
| 500 |
|
|
|a Date Revised 18.01.2019
|
| 500 |
|
|
|a published: Print-Electronic
|
| 500 |
|
|
|a Citation Status MEDLINE
|
| 520 |
|
|
|a Membrane proteins usually need to be extracted from their native environment and separated from other membrane components for in-depth in vitro characterization. The use of styrene/maleic acid (SMA) copolymers to solubilize membrane proteins and their surrounding lipids into bilayer nanodiscs is an attractive approach toward this goal. We have recently shown that a diisobutylene/maleic acid (DIBMA) copolymer similarly solubilizes model and cellular membranes but, unlike SMA(3:1), has a mild impact on lipid acyl-chain order and thermotropic phase behavior. Here, we used fluorescence spectroscopy, small-angle X-ray scattering, size-exclusion chromatography, dynamic light scattering, and 31P nuclear magnetic resonance spectroscopy to examine the self-association of DIBMA and its membrane-solubilization properties against lipids differing in acyl-chain length and saturation. Although DIBMA is less hydrophobic than commonly used SMA(3:1) and SMA(2:1) copolymers, it efficiently formed lipid-bilayer nanodiscs that decreased in size with increasing polymer/lipid ratio while maintaining the overall thickness of the membrane. DIBMA fractions of different molar masses were similarly efficient in solubilizing a saturated lipid. Coulomb screening at elevated ionic strength or reduced charge density on the polymer at low pH enhanced the solubilization efficiency of DIBMA. The free-energy penalty for transferring phospholipids from vesicular bilayers into nanodiscs became more unfavorable with increasing acyl-chain length and unsaturation. Altogether, these findings provide a rational framework for using DIBMA in membrane-protein research by shedding light on the effects of polymer and lipid properties as well as experimental conditions on membrane solubilization
|
| 650 |
|
4 |
|a Journal Article
|
| 650 |
|
4 |
|a Research Support, Non-U.S. Gov't
|
| 650 |
|
7 |
|a Alkenes
|2 NLM
|
| 650 |
|
7 |
|a Lipid Bilayers
|2 NLM
|
| 650 |
|
7 |
|a Maleates
|2 NLM
|
| 650 |
|
7 |
|a maleic acid
|2 NLM
|
| 650 |
|
7 |
|a 91XW058U2C
|2 NLM
|
| 650 |
|
7 |
|a diisobutylene
|2 NLM
|
| 650 |
|
7 |
|a N69L73ADVF
|2 NLM
|
| 700 |
1 |
|
|a Klingler, Johannes
|e verfasserin
|4 aut
|
| 700 |
1 |
|
|a Danielczak, Bartholomäus
|e verfasserin
|4 aut
|
| 700 |
1 |
|
|a Babalola, Jonathan Oyebamiji
|e verfasserin
|4 aut
|
| 700 |
1 |
|
|a Vargas, Carolyn
|e verfasserin
|4 aut
|
| 700 |
1 |
|
|a Pabst, Georg
|e verfasserin
|4 aut
|
| 700 |
1 |
|
|a Keller, Sandro
|e verfasserin
|4 aut
|
| 773 |
0 |
8 |
|i Enthalten in
|t Langmuir : the ACS journal of surfaces and colloids
|d 1992
|g 33(2017), 50 vom: 19. Dez., Seite 14378-14388
|w (DE-627)NLM098181009
|x 1520-5827
|7 nnns
|
| 773 |
1 |
8 |
|g volume:33
|g year:2017
|g number:50
|g day:19
|g month:12
|g pages:14378-14388
|
| 856 |
4 |
0 |
|u http://dx.doi.org/10.1021/acs.langmuir.7b03742
|3 Volltext
|
| 912 |
|
|
|a GBV_USEFLAG_A
|
| 912 |
|
|
|a SYSFLAG_A
|
| 912 |
|
|
|a GBV_NLM
|
| 912 |
|
|
|a GBV_ILN_22
|
| 912 |
|
|
|a GBV_ILN_350
|
| 912 |
|
|
|a GBV_ILN_721
|
| 951 |
|
|
|a AR
|
| 952 |
|
|
|d 33
|j 2017
|e 50
|b 19
|c 12
|h 14378-14388
|