Molecular testing of adult Pacific salmon and trout (Oncorhynchus spp.) for several RNA viruses demonstrates widespread distribution of piscine orthoreovirus in Alaska and Washington

Molecular testing of adult Pacific salmon and trout (Oncorhynchus spp.) for several RNA viruses demonstrates widespread distribution of piscine orthoreovirus in Alaska and Washington

© 2017 John Wiley & Sons Ltd.

Bibliographische Detailangaben
Veröffentlicht in:Journal of fish diseases. - 1998. - 41(2018), 2 vom: 15. Feb., Seite 347-355
1. Verfasser: Purcell, M K (VerfasserIn)
Weitere Verfasser: Powers, R L, Evered, J, Kerwin, J, Meyers, T R, Stewart, B, Winton, J R
Format: Online-Aufsatz
Sprache:English
Veröffentlicht: 2018
Zugriff auf das übergeordnete Werk:Journal of fish diseases
Schlagworte:Journal Article infectious salmon anaemia piscine myocarditis virus piscine orthoreovirus reverse transcription real-time PCR salmonid alphavirus surveillance RNA, Viral
Beschreibung
Zusammenfassung:© 2017 John Wiley & Sons Ltd.
This research was initiated in conjunction with a systematic, multiagency surveillance effort in the United States (U.S.) in response to reported findings of infectious salmon anaemia virus (ISAV) RNA in British Columbia, Canada. In the systematic surveillance study reported in a companion paper, tissues from various salmonids taken from Washington and Alaska were surveyed for ISAV RNA using the U.S.-approved diagnostic method, and samples were released for use in this present study only after testing negative. Here, we tested a subset of these samples for ISAV RNA with three additional published molecular assays, as well as for RNA from salmonid alphavirus (SAV), piscine myocarditis virus (PMCV) and piscine orthoreovirus (PRV). All samples (n = 2,252; 121 stock cohorts) tested negative for RNA from ISAV, PMCV, and SAV. In contrast, there were 25 stock cohorts from Washington and Alaska that had one or more individuals test positive for PRV RNA; prevalence within stocks varied and ranged from 2% to 73%. The overall prevalence of PRV RNA-positive individuals across the study was 3.4% (77 of 2,252 fish tested). Findings of PRV RNA were most common in coho (Oncorhynchus kisutch Walbaum) and Chinook (O. tshawytscha Walbaum) salmon
Beschreibung:Date Completed 06.07.2018
Date Revised 09.12.2020
published: Print-Electronic
GENBANK: KY369097, KY369126, KY379959, KR478633, KR478634
Citation Status MEDLINE
ISSN:1365-2761
DOI:10.1111/jfd.12740