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231225s2017 xx |||||o 00| ||eng c |
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|a 10.1093/jxb/erx362
|2 doi
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|a pubmed24n0927.xml
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|a (DE-627)NLM278221815
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|a (NLM)29155974
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|a DE-627
|b ger
|c DE-627
|e rakwb
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|a eng
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| 100 |
1 |
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|a Yang, Ting
|e verfasserin
|4 aut
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| 245 |
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|a Transcript profiling of a bitter variety of narrow-leafed lupin to discover alkaloid biosynthetic genes
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|c 2017
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
|b c
|2 rdamedia
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|a ƒa Online-Ressource
|b cr
|2 rdacarrier
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|a Date Completed 24.07.2018
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|a Date Revised 13.11.2018
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|a published: Print
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|a Citation Status MEDLINE
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|a © The Author 2017. Published by Oxford University Press on behalf of the Society for Experimental Biology.
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|a Lupins (Lupinus spp.) are nitrogen-fixing legumes that accumulate toxic alkaloids in their protein-rich beans. These anti-nutritional compounds belong to the family of quinolizidine alkaloids (QAs), which are of interest to the pharmaceutical and chemical industries. To unleash the potential of lupins as protein crops and as sources of QAs, a thorough understanding of the QA pathway is needed. However, only the first enzyme in the pathway, lysine decarboxylase (LDC), is known. Here, we report the transcriptome of a high-QA variety of narrow-leafed lupin (L. angustifolius), obtained using eight different tissues and two different sequencing technologies. In addition, we present a list of 33 genes that are closely co-expressed with LDC and that represent strong candidates for involvement in lupin alkaloid biosynthesis. One of these genes encodes a copper amine oxidase able to convert the product of LDC, cadaverine, into 1-piperideine, as shown by heterologous expression and enzyme assays. Kinetic analysis revealed a low KM value for cadaverine, supporting a role as the second enzyme in the QA pathway. Our transcriptomic data set represents a crucial step towards the discovery of enzymes, transporters, and regulators involved in lupin alkaloid biosynthesis
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|a Journal Article
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|a Alkaloid biosynthesis
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|a copper amine oxidase
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|a narrow-leafed lupin
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|a next-generation sequencing
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|a quinolizidine alkaloids
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| 650 |
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|a transcript profiling
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| 650 |
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|a Alkaloids
|2 NLM
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| 650 |
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|a Plant Proteins
|2 NLM
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| 650 |
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|a Quinolizidines
|2 NLM
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| 650 |
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|a Carboxy-Lyases
|2 NLM
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| 650 |
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7 |
|a EC 4.1.1.-
|2 NLM
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| 650 |
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|a lysine decarboxylase
|2 NLM
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| 650 |
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|a EC 4.1.1.18
|2 NLM
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| 700 |
1 |
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|a Nagy, Istvan
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Mancinotti, Davide
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Otterbach, Sophie Lisa
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Andersen, Trine Bundgaard
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Motawia, Mohammed Saddik
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Asp, Torben
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Geu-Flores, Fernando
|e verfasserin
|4 aut
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| 773 |
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|i Enthalten in
|t Journal of experimental botany
|d 1985
|g 68(2017), 20 vom: 28. Nov., Seite 5527-5537
|w (DE-627)NLM098182706
|x 1460-2431
|7 nnns
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| 773 |
1 |
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|g volume:68
|g year:2017
|g number:20
|g day:28
|g month:11
|g pages:5527-5537
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| 856 |
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|u http://dx.doi.org/10.1093/jxb/erx362
|3 Volltext
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|d 68
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|h 5527-5537
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