A role for the gene regulatory module microRNA172/TARGET OF EARLY ACTIVATION TAGGED 1/FLOWERING LOCUS T (miRNA172/TOE1/FT) in the feeding sites induced by Meloidogyne javanica in Arabidopsis thaliana

A role for the gene regulatory module microRNA172/TARGET OF EARLY ACTIVATION TAGGED 1/FLOWERING LOCUS T (miRNA172/TOE1/FT) in the feeding sites induced by Meloidogyne javanica in Arabidopsis thaliana

© 2017 The Authors. New Phytologist © 2017 New Phytologist Trust.

Bibliographische Detailangaben
Veröffentlicht in:The New phytologist. - 1989. - 217(2018), 2 vom: 06. Jan., Seite 813-827
1. Verfasser: Díaz-Manzano, Fernando E (VerfasserIn)
Weitere Verfasser: Cabrera, Javier, Ripoll, Juan-José, Del Olmo, Iván, Andrés, Mari Fe, Silva, Ana Cláudia, Barcala, Marta, Sánchez, María, Ruíz-Ferrer, Virginia, de Almeida-Engler, Janice, Yanofsky, Martin F, Piñeiro, Manuel, Jarillo, Jose Antonio, Fenoll, Carmen, Escobar, Carolina
Format: Online-Aufsatz
Sprache:English
Veröffentlicht: 2018
Zugriff auf das übergeordnete Werk:The New phytologist
Schlagworte:Journal Article Research Support, Non-U.S. Gov't Arabidopsis thaliana APETALA2 (AP2) Meloidogyne spp. flowering giant cells microRNA172 repression Arabidopsis Proteins mehr... FT protein, Arabidopsis Indoleacetic Acids MicroRNAs Mirn172 microRNA, Arabidopsis Glucuronidase EC 3.2.1.31
Beschreibung
Zusammenfassung:© 2017 The Authors. New Phytologist © 2017 New Phytologist Trust.
Root knot nematodes (RKNs) penetrate into the root vascular cylinder, triggering morphogenetic changes to induce galls, de novo formed 'pseudo-organs' containing several giant cells (GCs). Distinctive gene repression events observed in early gall/GCs development are thought to be mediated by post-transcriptional silencing via microRNAs (miRNAs), a process that is far from being fully characterized. Arabidopsis thaliana backgrounds with altered activities based on target 35S::MIMICRY172 (MIM172), 35S::TARGET OF EARLY ACTIVATION TAGGED 1 (TOE1)-miR172-resistant (35S::TOE1R ) and mutant (flowering locus T-10 (ft-10)) lines were used for functional analysis of nematode infective and reproductive parameters. The GUS-reporter lines, MIR172A-E::GUS, treated with auxin (IAA) and an auxin-inhibitor (a-(phenyl ethyl-2-one)-indole-3-acetic acid (PEO-IAA)), together with the MIR172C AuxRE::GUS line with two mutated auxin responsive elements (AuxREs), were assayed for nematode-dependent gene expression. Arabidopsis thaliana backgrounds with altered expression of miRNA172, TOE1 or FT showed lower susceptibility to the RKNs and smaller galls and GCs. MIR172C-D::GUS showed restricted promoter activity in galls/GCs that was regulated by auxins through auxin-responsive factors. IAA induced their activity in galls while PEO-IAA treatment and mutations in AuxRe motifs abolished it. The results showed that the regulatory module miRNA172/TOE1/FT plays an important role in correct GCs and gall development, where miRNA172 is modulated by auxins
Beschreibung:Date Completed 12.09.2019
Date Revised 30.09.2020
published: Print-Electronic
GENBANK: NM_001247718, AF325506.1
Citation Status MEDLINE
ISSN:1469-8137
DOI:10.1111/nph.14839