WRINKLED1 and ACYL-COA:DIACYLGLYCEROL ACYLTRANSFERASE1 regulate tocochromanol metabolism in Arabidopsis

WRINKLED1 and ACYL-COA:DIACYLGLYCEROL ACYLTRANSFERASE1 regulate tocochromanol metabolism in Arabidopsis

© 2017 The Authors. New Phytologist © 2017 New Phytologist Trust.

Bibliographische Detailangaben
Veröffentlicht in:The New phytologist. - 1979. - 217(2018), 1 vom: 01. Jan., Seite 245-260
1. Verfasser: Pellaud, Sébastien (VerfasserIn)
Weitere Verfasser: Bory, Alexandre, Chabert, Valentin, Romanens, Joëlle, Chaisse-Leal, Laurie, Doan, Anh Vu, Frey, Lucas, Gust, Andrea, Fromm, Katharina M, Mène-Saffrané, Laurent
Format: Online-Aufsatz
Sprache:English
Veröffentlicht: 2018
Zugriff auf das übergeordnete Werk:The New phytologist
Schlagworte:Journal Article diacylglycerol acyltransferase1 (dgat1) nutrigenomics tocomonoenol tocopherol vitamin E wrinkled1 (wri1) 1,2-diacylglycerol Acyl Coenzyme A Arabidopsis Proteins mehr... Chromans Diglycerides Tocotrienols Transcription Factors WRINKLED1 protein, Arabidopsis Vitamin E 1406-18-4 plastochromanol 8 4382-43-8 DGAT1 protein, Arabidopsis EC 2.3.1.20 Diacylglycerol O-Acyltransferase Alkyl and Aryl Transferases EC 2.5.- HPT1 protein, Arabidopsis Intramolecular Transferases EC 5.4.- tocopherol cyclase EC 5.4.99.- Tocopherols R0ZB2556P8
LEADER 01000naa a22002652 4500
001 NLM277722829
003 DE-627
005 20231225014914.0
007 cr uuu---uuuuu
008 231225s2018 xx |||||o 00| ||eng c
024 7 |a 10.1111/nph.14856  |2 doi 
028 5 2 |a pubmed24n0925.xml 
035 |a (DE-627)NLM277722829 
035 |a (NLM)29105089 
040 |a DE-627  |b ger  |c DE-627  |e rakwb 
041 |a eng 
100 1 |a Pellaud, Sébastien  |e verfasserin  |4 aut 
245 1 0 |a WRINKLED1 and ACYL-COA:DIACYLGLYCEROL ACYLTRANSFERASE1 regulate tocochromanol metabolism in Arabidopsis 
264 1 |c 2018 
336 |a Text  |b txt  |2 rdacontent 
337 |a ƒaComputermedien  |b c  |2 rdamedia 
338 |a ƒa Online-Ressource  |b cr  |2 rdacarrier 
500 |a Date Completed 27.07.2018 
500 |a Date Revised 30.09.2020 
500 |a published: Print-Electronic 
500 |a Citation Status MEDLINE 
520 |a © 2017 The Authors. New Phytologist © 2017 New Phytologist Trust. 
520 |a Photosynthetic organisms such as plants, algae and some cyanobacteria synthesize tocochromanols, a group of compounds that encompasses tocopherols and tocotrienols and that exhibits vitamin E activity in animals. While most vitamin E biosynthetic genes have been identified in plant genomes, regulatory genes controlling tocopherol accumulation are currently unknown. We isolated by forward genetics Arabidopsis enhanced vitamin E (eve) mutants that overaccumulate the classic tocopherols and plastochromanol-8, and a tocochromanol unknown in this species. We mapped eve1 and eve4, and identified the unknown Arabidopsis tocochromanol by using a combination of analytical tools. In addition, we determined its biosynthetic pathway with a series of tocochromanol biosynthetic mutants and transgenic lines. eve1 and eve4 are two seed lipid mutants affecting the WRINKLED1 (WRI1) and ACYL-COA:DIACYLGLYCEROL ACYLTRANSFERASE1 (DGAT1) genes, respectively. The unknown tocochromanol is 11'-12' γ-tocomonoenol, whose biosynthesis is VITAMIN E 1 (VTE1) - and VTE2-dependent and is initiated by the condensation of homogentisate (HGA) and tetrahydrogeranylgeranyl pyrophosphate. This study identifies the first two regulatory genes, WRI1 and DGAT1, that control the synthesis of all tocochromanol forms in seeds, and shows the existence of a metabolic trade-off between lipid and tocochromanol metabolisms. Moreover, it shows that Arabidopsis possesses a tocomonoenol biosynthetic pathway that competes with tocopherol synthesis 
650 4 |a Journal Article 
650 4 |a diacylglycerol acyltransferase1 (dgat1) 
650 4 |a nutrigenomics 
650 4 |a tocomonoenol 
650 4 |a tocopherol 
650 4 |a vitamin E 
650 4 |a wrinkled1 (wri1) 
650 7 |a 1,2-diacylglycerol  |2 NLM 
650 7 |a Acyl Coenzyme A  |2 NLM 
650 7 |a Arabidopsis Proteins  |2 NLM 
650 7 |a Chromans  |2 NLM 
650 7 |a Diglycerides  |2 NLM 
650 7 |a Tocotrienols  |2 NLM 
650 7 |a Transcription Factors  |2 NLM 
650 7 |a WRINKLED1 protein, Arabidopsis  |2 NLM 
650 7 |a Vitamin E  |2 NLM 
650 7 |a 1406-18-4  |2 NLM 
650 7 |a plastochromanol 8  |2 NLM 
650 7 |a 4382-43-8  |2 NLM 
650 7 |a DGAT1 protein, Arabidopsis  |2 NLM 
650 7 |a EC 2.3.1.20  |2 NLM 
650 7 |a Diacylglycerol O-Acyltransferase  |2 NLM 
650 7 |a EC 2.3.1.20  |2 NLM 
650 7 |a Alkyl and Aryl Transferases  |2 NLM 
650 7 |a EC 2.5.-  |2 NLM 
650 7 |a HPT1 protein, Arabidopsis  |2 NLM 
650 7 |a EC 2.5.-  |2 NLM 
650 7 |a Intramolecular Transferases  |2 NLM 
650 7 |a EC 5.4.-  |2 NLM 
650 7 |a tocopherol cyclase  |2 NLM 
650 7 |a EC 5.4.99.-  |2 NLM 
650 7 |a Tocopherols  |2 NLM 
650 7 |a R0ZB2556P8  |2 NLM 
700 1 |a Bory, Alexandre  |e verfasserin  |4 aut 
700 1 |a Chabert, Valentin  |e verfasserin  |4 aut 
700 1 |a Romanens, Joëlle  |e verfasserin  |4 aut 
700 1 |a Chaisse-Leal, Laurie  |e verfasserin  |4 aut 
700 1 |a Doan, Anh Vu  |e verfasserin  |4 aut 
700 1 |a Frey, Lucas  |e verfasserin  |4 aut 
700 1 |a Gust, Andrea  |e verfasserin  |4 aut 
700 1 |a Fromm, Katharina M  |e verfasserin  |4 aut 
700 1 |a Mène-Saffrané, Laurent  |e verfasserin  |4 aut 
773 0 8 |i Enthalten in  |t The New phytologist  |d 1979  |g 217(2018), 1 vom: 01. Jan., Seite 245-260  |w (DE-627)NLM09818248X  |x 1469-8137  |7 nnns 
773 1 8 |g volume:217  |g year:2018  |g number:1  |g day:01  |g month:01  |g pages:245-260 
856 4 0 |u http://dx.doi.org/10.1111/nph.14856  |3 Volltext 
912 |a GBV_USEFLAG_A 
912 |a SYSFLAG_A 
912 |a GBV_NLM 
912 |a GBV_ILN_350 
951 |a AR 
952 |d 217  |j 2018  |e 1  |b 01  |c 01  |h 245-260