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231225s2017 xx |||||o 00| ||eng c |
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|a 10.1093/jxb/erx288
|2 doi
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|a pubmed24n1334.xml
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|a (DE-627)NLM277170788
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|a (NLM)29048567
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|a DE-627
|b ger
|c DE-627
|e rakwb
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|a eng
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|a Kilambi, Himabindu Vasuki
|e verfasserin
|4 aut
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|a Green-fruited Solanum habrochaites lacks fruit-specific carotenogenesis due to metabolic and structural blocks
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|c 2017
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
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|2 rdamedia
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|a ƒa Online-Ressource
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|2 rdacarrier
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|a Date Completed 14.05.2018
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|a Date Revised 18.03.2024
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|a published: Print
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|a Citation Status MEDLINE
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|a © The Author 2017. Published by Oxford University Press on behalf of the Society for Experimental Biology.
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|a Members of the tomato clade exhibit a wide diversity in fruit color, but the mechanisms governing inter-species diversity of coloration are largely unknown. The carotenoid profiles, carotenogenic gene expression and proteome profiles of green-fruited Solanum habrochaites (SH), orange-fruited S. galapagense, and red-fruited S. pimpinellifolium were compared with cultivated tomato [S. lycopersicum cv. Ailsa Craig (SL)] to decipher the molecular basis of coloration diversity. Green-fruited SH, though it showed normal expression of chromoplast-specific phytoene synthase1 and lycopene β-cyclase genes akin to orange/red-fruited species, failed to accumulate lycopene and β-carotene. The SH phytoene synthase1 cDNA encoded an enzymatically active protein, whereas the lycopene β-cyclase cDNA was barely active. Consistent with its green-fruited nature, SH's fruits retained chloroplast structure and PSII activity, and had impaired chlorophyll degradation with high pheophorbide a levels. Comparison of the fruit proteomes with SL revealed retention of the proteome complement related to photosynthesis in SH. Targeted peptide monitoring revealed a low abundance of key carotenogenic and sequestration proteins in SH compared with tomato. The green-fruitedness of SH appears to stem from blocks at several critical steps regulating fruit-specific carotenogenesis namely the absence of chloroplast to chromoplast transformation, block in carotenoid biosynthesis, and a dearth of carotenoid sequestering proteins
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|a Journal Article
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|a Research Support, Non-U.S. Gov't
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|a Carotenoid biosynthesis
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|a carotenoid sequestration
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|a fruit color
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|a gene expression
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|a proteome analysis
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|a tomato clade
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|a Pigments, Biological
|2 NLM
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|a Plant Proteins
|2 NLM
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| 650 |
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|a Proteome
|2 NLM
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| 650 |
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|a Carotenoids
|2 NLM
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|a 36-88-4
|2 NLM
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|a Manda, Kalyani
|e verfasserin
|4 aut
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|a Rai, Avanish
|e verfasserin
|4 aut
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|a Charakana, Chaitanya
|e verfasserin
|4 aut
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|a Bagri, Jayram
|e verfasserin
|4 aut
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|a Sharma, Rameshwar
|e verfasserin
|4 aut
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|a Sreelakshmi, Yellamaraju
|e verfasserin
|4 aut
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| 773 |
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|i Enthalten in
|t Journal of experimental botany
|d 1985
|g 68(2017), 17 vom: 13. Okt., Seite 4803-4819
|w (DE-627)NLM098182706
|x 1460-2431
|7 nnns
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| 773 |
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|g volume:68
|g year:2017
|g number:17
|g day:13
|g month:10
|g pages:4803-4819
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|u http://dx.doi.org/10.1093/jxb/erx288
|3 Volltext
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|d 68
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|e 17
|b 13
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|h 4803-4819
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